Detection of homologous recombination between yeast artificial chromosomes with overlapping inserts

We have developed a system which facilitates the detection of recombination between YeastArtificial Chromosomes (YAC's) carrying homologous inserts. The system consists of a classical YAC vector, a new YAC vector and two appropriately labelled yeast strains of opposite mating type. The new YAC...

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Bibliographic Details
Published in:Nucleic acids research 1991-03, Vol.19 (5), p.997-1000
Main Authors: Cellini, A, Lacatena, R.M, Tocchini-Valentini, G.P
Format: Article
Language:English
Subjects:
Bacteriophage lambda - genetics
Biological and medical sciences
chromosomes
Chromosomes, Fungal
Cloning, Molecular
Fundamental and applied biological sciences. Psychology
Genes. Genome
Genetic Vectors
homologous recombination
Meiosis
Molecular and cellular biology
Molecular genetics
Phenotype
Recombination, Genetic
Sequence Homology, Nucleic Acid
vectors
yac
yac vectors
yeasts
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Summary:We have developed a system which facilitates the detection of recombination between YeastArtificial Chromosomes (YAC's) carrying homologous inserts. The system consists of a classical YAC vector, a new YAC vector and two appropriately labelled yeast strains of opposite mating type. The new YAC vector differs in markers from the canonical YAC vector. To test whether homologous recombination takes place, phage lambda DNA was cloned in the two vectors to provide a region of homology. The two constructs were then introduced into yeast strains of opposite mating type in which the endogenous genes for the selective markers present in the vectors are not expressed. Artificial chromosomes obtained by meiotic recombination are detected in the spores resulting from the mating.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/19.5.997