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Detection of homologous recombination between yeast artificial chromosomes with overlapping inserts

We have developed a system which facilitates the detection of recombination between YeastArtificial Chromosomes (YAC's) carrying homologous inserts. The system consists of a classical YAC vector, a new YAC vector and two appropriately labelled yeast strains of opposite mating type. The new YAC...

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Published in:	Nucleic acids research 1991-03, Vol.19 (5), p.997-1000
Main Authors:	Cellini, A, Lacatena, R.M, Tocchini-Valentini, G.P
Format:	Article
Language:	English
Subjects:	Bacteriophage lambda - genetics Biological and medical sciences chromosomes Chromosomes, Fungal Cloning, Molecular Fundamental and applied biological sciences. Psychology Genes. Genome Genetic Vectors homologous recombination Meiosis Molecular and cellular biology Molecular genetics Phenotype Recombination, Genetic Sequence Homology, Nucleic Acid vectors yac yac vectors yeasts
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container_title	Nucleic acids research
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creator	Cellini, A Lacatena, R.M Tocchini-Valentini, G.P
description	We have developed a system which facilitates the detection of recombination between YeastArtificial Chromosomes (YAC's) carrying homologous inserts. The system consists of a classical YAC vector, a new YAC vector and two appropriately labelled yeast strains of opposite mating type. The new YAC vector differs in markers from the canonical YAC vector. To test whether homologous recombination takes place, phage lambda DNA was cloned in the two vectors to provide a region of homology. The two constructs were then introduced into yeast strains of opposite mating type in which the endogenous genes for the selective markers present in the vectors are not expressed. Artificial chromosomes obtained by meiotic recombination are detected in the spores resulting from the mating.
doi_str_mv	10.1093/nar/19.5.997
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Genome</subject><subject>Genetic Vectors</subject><subject>homologous recombination</subject><subject>Meiosis</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Phenotype</subject><subject>Recombination, Genetic</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>vectors</subject><subject>yac</subject><subject>yac vectors</subject><subject>yeasts</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNqFkTFvFDEQhS0ECkego0VsAxV3Gdtr77qgQCEBpEgUkNqa847vjHbXh-1LlH-P4U4EqhSWi_e95_E8xl5yWHEw8mzGdMbNSq2M6R6xBZdaLFujxWO2AAlqyaHtn7JnOf8A4C1X7Qk74b3QRvEFcx-pkCshzk30zTZOcYybuM9NIhendZjxj7amcks0N3eEuTSYSvDBBRwbt03Vk-NEubkNZdvEG0oj7nZh3jRhzpRKfs6eeBwzvTjep-z68uL7-efl1ddPX84_XC1da9qyHLrWazBGdp463zsUAMqAEm3fa68AlR4G4kaCJ-g1CocwaKE5GqnXbSdP2ftD7m6_nmhwNJeEo92lMGG6sxGD_V-Zw9Zu4o2VUnYdr_63R3-KP_eUi51CdjSOOFNdie1BSWk0PAjyuv0a2T8MallLM6KC7w6gSzHnRP7v1Bzs75ZtbbnmWmWroeKv_v3pPXyotepvjjpmh6NPOLuQ7zGjJa9vV-71gfMYLW5SZa6_CeASeCdFPfIXEu275w</recordid><startdate>19910311</startdate><enddate>19910311</enddate><creator>Cellini, A</creator><creator>Lacatena, R.M</creator><creator>Tocchini-Valentini, G.P</creator><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19910311</creationdate><title>Detection of homologous recombination between yeast artificial chromosomes with overlapping inserts</title><author>Cellini, A ; Lacatena, R.M ; Tocchini-Valentini, G.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-d74f609937fe7f8ca200590524886f50a56dde1930fe086a2ca0d6261a936b473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Bacteriophage lambda - genetics</topic><topic>Biological and medical sciences</topic><topic>chromosomes</topic><topic>Chromosomes, Fungal</topic><topic>Cloning, Molecular</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes. Genome</topic><topic>Genetic Vectors</topic><topic>homologous recombination</topic><topic>Meiosis</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Phenotype</topic><topic>Recombination, Genetic</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>vectors</topic><topic>yac</topic><topic>yac vectors</topic><topic>yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cellini, A</creatorcontrib><creatorcontrib>Lacatena, R.M</creatorcontrib><creatorcontrib>Tocchini-Valentini, G.P</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cellini, A</au><au>Lacatena, R.M</au><au>Tocchini-Valentini, G.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of homologous recombination between yeast artificial chromosomes with overlapping inserts</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1991-03-11</date><risdate>1991</risdate><volume>19</volume><issue>5</issue><spage>997</spage><epage>1000</epage><pages>997-1000</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>We have developed a system which facilitates the detection of recombination between YeastArtificial Chromosomes (YAC's) carrying homologous inserts. 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source	PubMed Central; Oxford University Press:Jisc Collections:Oxford Journal Archive: Access period 2024-2025
subjects	Bacteriophage lambda - genetics Biological and medical sciences chromosomes Chromosomes, Fungal Cloning, Molecular Fundamental and applied biological sciences. Psychology Genes. Genome Genetic Vectors homologous recombination Meiosis Molecular and cellular biology Molecular genetics Phenotype Recombination, Genetic Sequence Homology, Nucleic Acid vectors yac yac vectors yeasts
title	Detection of homologous recombination between yeast artificial chromosomes with overlapping inserts
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