Adjacent pol II and pol III promoters: transcription of the yeast retrotransposon Ty3 and a target tRNA gene

The Saccharomyces cerevisiae retrotransposon Ty3 integrates 16 to 19 basepairs upstream of tRNA genes in a region where sequences have been shown to affect the expression of tRNA genes in vivo and in vitro. Sigma, the isolated long terminal repeat of Ty3, is also found in this region. The purpose of...

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Bibliographic Details
Published in:Nucleic acids research 1991-03, Vol.19 (6), p.1317-1324
Main Authors: Kinsey, P.T, Sandmeyer, S.B
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Blotting, Northern
DNA Transposable Elements - genetics
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Fungal
Genes, Fungal
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Plasmids
Promoter Regions, Genetic
RNA Polymerase II - genetics
RNA Polymerase III - genetics
RNA, Transfer, Tyr - genetics
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
transcription (genetics)
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
transfer RNA
transposons
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Summary:The Saccharomyces cerevisiae retrotransposon Ty3 integrates 16 to 19 basepairs upstream of tRNA genes in a region where sequences have been shown to affect the expression of tRNA genes in vivo and in vitro. Sigma, the isolated long terminal repeat of Ty3, is also found in this region. The purpose of these experiments was to elucidate the effects of Ty3 and sigma expression on that of an associated SUP2 tRNATyr gene in vivo. SUP2 pre-tRNA levels were moderately increased when SUP2 was associated with Ty3 or sigma in either orientation. These increases were independent of Ty3 or sigma promoter activity. The presence of Ty3 or sigma also increased the usage of a minor SUP2 transcription initiation site 2 basepairs upstream of the major initiation site and within the 5 basepair direct repeat flanking Ty3 and sigma. Transcription from an isolated sigma directed toward the tRNA gene was observed to extend through the tRNA gene. In contrast to the lack of an effect of sigma induction on pre-tRNATyr levels, levels of this sigma transcript were increased when the SUP2 promoter was inactivated by a single basepair mutation.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/19.6.1317