Interactions of nucleic acids with distamycins. Binding of Dst-3 to d(CGTTTAAACG)2 and d(CGTACGTACG)2

The binding between Distamycin 3 and the palindromic duplexes d(CGTTTAAACG)2 and d(CGTACGTACG)2 was investigated by two independent techniques: UV-Vis absorption in the Job's plot approach and Induced Circular Dichroism. Both decamers bind two molecules of peptide per duplex, with close overall...

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Bibliographic Details
Published in:Nucleic acids research 1991-04, Vol.19 (7), p.1695-1698
Main Authors: CAPOBIANCO, M. L, COLONNA, F. P, FORNI, A, GARBESI, A, IOTTI, S, MORETTI, I, SAMORI, B, TONDELLI, L
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Circular Dichroism
Distamycins - metabolism
Fundamental and applied biological sciences. Psychology
Interactions. Associations
Intermolecular phenomena
Molecular biophysics
Molecular Sequence Data
Nucleic Acid Heteroduplexes
Oligodeoxyribonucleotides - chemistry
Oligodeoxyribonucleotides - metabolism
Spectrophotometry, Ultraviolet
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Summary:The binding between Distamycin 3 and the palindromic duplexes d(CGTTTAAACG)2 and d(CGTACGTACG)2 was investigated by two independent techniques: UV-Vis absorption in the Job's plot approach and Induced Circular Dichroism. Both decamers bind two molecules of peptide per duplex, with close overall affinities. This result indicates that a row of six A:T base pairs can accommodate two molecules of drug and that the minimal binding site of Distamycin 3 can consist of just two A:T base pairs.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/19.7.1695