In vivo photoacoustic time-of-flight velocity measurement of single cells and nanoparticles

Optical techniques for in vivo measurement of blood flow velocity are not quite applicable for determination of velocity of individual cells or nanoparticles. Here, we describe a photoacoustic time-of-flight method to measure the velocity of individual absorbing objects by using single and multiple...

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Bibliographic Details
Published in:Optics letters 2011-10, Vol.36 (20), p.4086-4088
Main Authors: SARIMOLLAOGLU, M, NEDOSEKIN, D. A, SIMANOVSKY, Y, GALANZHA, E. I, ZHAROV, V. P
Format: Article
Language:English
Subjects:
Acoustics
Animals
Arterioles - physiology
Biological and medical sciences
Blood Flow Velocity - physiology
Cell Count
Cell Line
Flow Cytometry - methods
Fundamental and applied biological sciences. Psychology
General aspects, investigation technics, apparatus
Gold - analysis
Humans
Image Interpretation, Computer-Assisted - methods
Lasers
Metal Nanoparticles - analysis
Mice
Neoplastic Cells, Circulating
Particle Size
Phantoms, Imaging
Tissues, organs and organisms biophysics
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Summary:Optical techniques for in vivo measurement of blood flow velocity are not quite applicable for determination of velocity of individual cells or nanoparticles. Here, we describe a photoacoustic time-of-flight method to measure the velocity of individual absorbing objects by using single and multiple laser beams. Its capability was demonstrated in vitro on blood vessel phantom and in vivo on an animal (mouse) model for estimating velocity of gold nanorods, melanin nanoparticles, erythrocytes, leukocytes, and circulating tumor cells in the broad range of flow velocity from 0.1 mm/s to 14 cm/s. Object velocity can be used to identify single cells circulating at different velocities or cell aggregates and to determine a cell's location in a vessel cross-section.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.36.004086