Intron 5 alpha of the COXI gene of yeast mitochondrial DNA is a mobile group I intron

We have found that intron 5 alpha of the COXI gene (al5 alpha) of yeast mtDNA is a mobile group I intron in crosses between strains having or lacking the intron. We have demonstrated the following hallmarks of that process: 1) co-conversion of flanking optional intron markers; 2) mutations that trun...

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Bibliographic Details
Published in:Nucleic acids research 1992-08, Vol.20 (15), p.4069-4076
Main Authors: Moran, J.V, Wernette, C.M, Mecklenburg, K.L, Butow, R.A, Perlman, P.S
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Crosses, Genetic
cytochrome-c oxidase
DNA Transposable Elements - genetics
DNA, Fungal - genetics
DNA, Mitochondrial - genetics
Electron Transport Complex IV - genetics
endonucleases
Endonucleases - genetics
Fundamental and applied biological sciences. Psychology
Genes. Genome
introns
Introns - genetics
Mitochondria - enzymology
mitochondrial DNA
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
mutation
Mutation - genetics
nucleases
Plasmids - genetics
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
structural genes
transposons
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Summary:We have found that intron 5 alpha of the COXI gene (al5 alpha) of yeast mtDNA is a mobile group I intron in crosses between strains having or lacking the intron. We have demonstrated the following hallmarks of that process: 1) co-conversion of flanking optional intron markers; 2) mutations that truncate the intron open reading frame block intron mobility; and 3) the intron open reading frame encodes an endonuclease activity that is required for intron movement. The endonuclease activity, termed I-Sce IV, cleaves the COXI allele lacking al5 alpha near the site of intron insertion, making a four-base staggered cut with 3' OH overhangs. Three cloned DNAs derived from different forms of the COXI gene, which differ in primary sequence at up to seven nucleotides around the cleavage site, are all good substrates for in vitro I-Sce IV cleavage activity. Two of the strains from which these substrates were derived were tested in crosses and are comparably efficient as al5 alpha recipients. When compared with omega mobility occurring simultaneously in one cross, al5 alpha is less efficient as a mobile element.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/20.15.4069