Yeast RNC1 encodes a chimeric protein, RhoNUC, with a human rho motif and deoxyribonuclease activity

The yeast Saccharomyces cerevisiae contains an endoexonuclease yNucR that has been implicated in both recombination and repair. We describe the isolation and characterization of the corresponding gene. Within the predicted N-terminal half of the protein there is extensive homology (approximately 50%...

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Bibliographic Details
Published in:Nucleic acids research 1992-10, Vol.20 (19), p.5215-5221
Main Authors: Chow, Terry Y.-K., Perkins, Edward L., Resnick, Michael A.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Blotting, Northern
Deoxyribonucleases - genetics
Deoxyribonucleases - metabolism
DNA, Fungal - genetics
DNA, Fungal - isolation & purification
Escherichia coli
Fundamental and applied biological sciences. Psychology
Genes, Fungal
Genes, ras
Genes. Genome
GTP-Binding Proteins - genetics
GTP-Binding Proteins - metabolism
Humans
Methyl Methanesulfonate - pharmacology
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Recombinant Fusion Proteins - metabolism
Rho Factor - genetics
Rho Factor - metabolism
RNA, Fungal - genetics
RNA, Fungal - isolation & purification
Saccharomyces cerevisiae
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - radiation effects
Saccharomyces cerevisiae Proteins
Sequence Homology, Amino Acid
tRNA Methyltransferases
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Description
Summary:The yeast Saccharomyces cerevisiae contains an endoexonuclease yNucR that has been implicated in both recombination and repair. We describe the isolation and characterization of the corresponding gene. Within the predicted N-terminal half of the protein there is extensive homology (approximately 50%) with human rho genes, which are related to the ras oncogene, particularly in the proposed GTP-binding region. The C-terminal region, which is related to the Escherichia coli recC protein, presumably encodes the endoexonuclease activity. The yNucR may thus represent a new class of GTP-bindlng proteins. Because of the chimeric nature of the polypeptide, this protein is renamed RhoNUC (rather than the original yNucR) and the gene is RNC1 for Rho-associated-NuClease. Over expression of the gene leads to altered cell growth and nuclear morphology. We propose that the gene plays an important role in cell development as well as DNA repair/recombination.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/20.19.5215