Characterization of Chlorella virus PBCV-1 CviAII restriction and modification system

• Published in: Nucleic acids research 1992-10, Vol.20 (20), p.5351-5356
• Main Authors: Zhang, Y, Nelson, M, Nietfeldt, J.W, Burbank, D.E, Van Etten, J.L
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; amino acid sequences; Analytical, structural and metabolic biochemistry; Base Sequence; Biological and medical sciences; Chlorella; Chlorella - enzymology; Chlorella - genetics; Chlorella virus; Cloning, Molecular; Deoxyribonucleases, Type II Site-Specific - chemistry; Deoxyribonucleases, Type II Site-Specific - genetics; Deoxyribonucleases, Type II Site-Specific - metabolism; DNA; dna methyltransferase; dna modification; enzyme activity; Enzymes and enzyme inhibitors; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; genbank/m86639; Genes, Viral - genetics; Genomic Library; Hydrolases; Methyltransferases - chemistry; Methyltransferases - genetics; Methyltransferases - metabolism; Molecular Sequence Data; nucleotide sequences; plant viruses; restriction endonucleases; restriction modification; Site-Specific DNA-Methyltransferase (Adenine-Specific) - chemistry; Site-Specific DNA-Methyltransferase (Adenine-Specific) - genetics; Site-Specific DNA-Methyltransferase (Adenine-Specific) - metabolism; structural genes; transferases; Viral Proteins - chemistry; Viral Proteins - genetics; Viral Proteins - metabolism; Viral Structural Proteins - genetics; Viruses - enzymology; Viruses - genetics
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/20.20.5351

A second DNA site-specific (restriction) endonuclease (R.CviAII) and its cognate adenine DNA methyltransferase (M.CviAII) were isolated from virus PBCV-1 infected Chlorella strain NC64A cells. R.CviAII, a heteroschizomer of the bacterial restriction endonuclease NlaIII, recognizes the sequence CATG, and does not cleave C(m)ATG sequences. However, unlike NlaIII, which cleaves after the G and does not cleave either C(m)ATG or (m)CATG sequences, CviAII cleaves between the C and A and is unaffected by (m)CATG methylation. The M.CviAII and R.CviAII genes were cloned and their DNA sequences were determined. These genes are tandemly arranged head-to-tail such that the TAA termination codon of the M.CviAII methyltransferase gene overlaps the ATG translational start site of R.CviAII endonuclease. R.CviAII is the first chlorella virus site-specific endonuclease gene to be cloned and sequenced.