The stability of different forms of double-stranded decoy DNA in serum and nuclear extracts

We have previously shown that transcription factors that bind to standard ds DNA bind with undiminished affinity to equivalent hairpin and dumbbell sequences. Since ds sequences in hairpin or dumbbell form cannot undergo strand separation, they should be advantageous as decoys. We compare the stabil...

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Bibliographic Details
Published in:Nucleic acids research 1992-11, Vol.20 (21), p.5857-5858
Main Authors: Chu, Barbara C.F., Orgel, Leslie E.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Base Sequence
Biological and medical sciences
Cell Nucleus - metabolism
DNA - blood
DNA - metabolism
Dna, deoxyribonucleoproteins
Exobiology
Fundamental and applied biological sciences. Psychology
HeLa Cells
Humans
Molecular Sequence Data
Nucleic Acid Conformation
Nucleic acids
Space life sciences
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Summary:We have previously shown that transcription factors that bind to standard ds DNA bind with undiminished affinity to equivalent hairpin and dumbbell sequences. Since ds sequences in hairpin or dumbbell form cannot undergo strand separation, they should be advantageous as decoys. We compare the stabilities of each of these forms of ds DNA in human serum and HeLa cell nuclear extracts.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/20.21.5857