Silencing of P2Y2 receptors reduces intraocular pressure in New Zealand rabbits

BACKGROUND AND PURPOSE P2 receptors are involved in the regulation of ocular physiological processes like intraocular pressure (IOP). In the present study, the involvement of P2Y2 receptors in the hypertensive effect of nucleotides was investigated by use of antagonists and of a siRNA designed for t...

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Bibliographic Details
Published in:British journal of pharmacology 2012-02, Vol.165 (4b), p.1163-1172
Main Authors: Martin‐Gil, Alba, de Lara, María Jesús Perez, Crooke, Almudena, Santano, Concepción, Peral, Assumpta, Pintor, Jesus
Format: Article
Language:English
Subjects:
eye
Glaucoma
intraocular pressure
P2Y2 receptor
Rabbits
Research Papers
siRNA
uridine nucleotides
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Summary:BACKGROUND AND PURPOSE P2 receptors are involved in the regulation of ocular physiological processes like intraocular pressure (IOP). In the present study, the involvement of P2Y2 receptors in the hypertensive effect of nucleotides was investigated by use of antagonists and of a siRNA designed for the P2Y2 receptor. EXPERIMENTAL APPROACH Agonists of the P2Y2 receptor a as well as P2 antagonists were applied to eyes of New Zealand rabbits, and the changes in IOP were followed for up to 6 h. Cloning of the P2Y2 receptor cDNA was done using a combination of degenerate reverse transcription PCR (RT‐PCR) and rapid amplification of cDNA ends (RACE). siRNA was synthesized and tested by immunohistochemistry. KEY RESULTS Single doses of 2‐thioUTP, UTP‐γ‐S and UTP increased IOP. This behaviour was concentration‐dependent and partially antagonized by reactive blue 2. Silencing the P2Y2 receptor was observed in the ciliary body by immunohistochemistry labelling, where a reduction in the immunofluorescence was observed. This reduction in the expression of the P2Y2 receptor was concomitant with a reduction in IOP, which was measurable 24 h after treatment with the siRNA, maximal after 2 days, followed by a slow increase towards control values for the following 5 days. Application of the P2Y2 agonists after pretreatment of the animals with this siRNA did not produce any change in IOP. CONCLUSIONS AND IMPLICATIONS P2Y2 receptors increase IOP in New Zealand rabbits. The application of a siRNA for this receptor significantly reduced IOP, suggesting that this technology might be used for the treatment of glaucoma.
ISSN:0007-1188
1476-5381
DOI:10.1111/j.1476-5381.2011.01586.x