Purinergic receptors expressed in human skeletal muscle fibres

• Published in: Purinergic signalling 2012-06, Vol.8 (2), p.255-264
• Main Authors: Bornø, A., Ploug, T., Bune, L. T., Rosenmeier, J. B., Thaning, P.
• Format: Article
• Language: English
• Subjects: Adult; Biomedical and Life Sciences; Biomedicine; Biopsy; Cancer Research; Cell Membrane - metabolism; Confocal microscopy; Diabetes mellitus; Female; Gene Expression Regulation; Human Physiology; Humans; Intracellular Fluid - metabolism; Male; Metabolism; Middle Aged; Muscle Fibers, Skeletal - metabolism; Neurosciences; Pharmacology/Toxicology; Plasma membranes; Purine P2X receptors; Purine P2Y receptors; Purine receptors; Receptors, Purinergic - biosynthesis; Receptors, Purinergic P2 - biosynthesis; Receptors, Purinergic P2Y1 - biosynthesis; Sarcolemma; Signal transduction; Skeletal muscle; Vesicles
• ISSN: 1573-9538; 1573-9546
• DOI: 10.1007/s11302-011-9279-y

Purinergic receptors are present in most tissues and thought to be involved in various signalling pathways, including neural signalling, cell metabolism and local regulation of the microcirculation in skeletal muscles. The present study aims to determine the distribution and intracellular content of purinergic receptors in skeletal muscle fibres in patients with type 2 diabetes and age-matched controls. Muscle biopsies from vastus lateralis were obtained from six type 2 diabetic patients and seven age-matched controls. Purinergic receptors were analysed using light and confocal microscopy in immunolabelled transverse sections of muscle biopsies. The receptors P2Y 4 , P2Y 11 and likely P2X 1 were present intracellularly or in the plasma membrane of muscle fibres and were thus selected for further detailed morphological analysis. P2X 1 receptors were expressed in intracellular vesicles and sarcolemma. P2Y 4 receptors were present in sarcolemma. P2Y 11 receptors were abundantly and diffusely expressed intracellularly and were more explicitly expressed in type I than in type II fibres, whereas P2X 1 and P2Y 4 showed no fibre-type specificity. Both diabetic patients and healthy controls showed similar distribution of receptors. The current study demonstrates that purinergic receptors are located intracellularly in human skeletal muscle fibres. The similar cellular localization of receptors in healthy and diabetic subjects suggests that diabetes is not associated with an altered distribution of purinergic receptors in skeletal muscle fibres. We speculate that the intracellular localization of purinergic receptors may reflect a role in regulation of muscle metabolism; further studies are nevertheless needed to determine the function of the purinergic system in skeletal muscle cells.