Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation

• Published in: Evidence-based complementary and alternative medicine 2012-01, Vol.2012 (2012), p.1-8
• Main Authors: Rais, Mohd Mustafa, Syam, Suvitha, Moharam, Bushra Abdulkarim, Hadi, A. Hamid A., Chuen, Cheah Shiau, Ahmad, Syahida, Elhassan, Manal Mohamed, Mohan, Syam, Yagi, Sakina M. Ahmed, Abdul Majid, Amin M. S., Hassan, Loiy Elsir Ahmed, Abdel Wahab, Siddig Ibrahim, Narrima, Putri
• Format: Article
• Language: English
• Subjects: Adenocarcinoma; Antioxidants; Apoptosis; Assaying; Caspase; Caspase-3; Colon cancer; Colorectal cancer; Colorimetry; Cucurbitaceae; Cytokines; Cytotoxicity; Folk medicine; Free radicals; Hepatocytes; Inflammation; Inflammatory bowel disease; Interferon; Kinases; Lipopolysaccharides; Liver; Liver cancer; Macrophages; Medical research; Medicinal plants; NG-Nitroarginine methyl ester; Nitric oxide; Nitrogen; Nitrous oxide; Pharmacology; Reactive nitrogen species; Reactive oxygen species; Ribonucleic acid; RNA; Rodents; Signal transduction; Tumors; γ-Interferon
• ISSN: 1741-427X; 1741-4288
• DOI: 10.1155/2012/490136

Emerging evidence suggests that reactive oxygen (ROS) and nitrogen (RNS) species can contribute to diverse signalling pathways of inflammatory and tumour cells. Cucurbitacins are a group of highly oxygenated triterpenes. Many plants used in folk medicine to treat cancer have been found to contain cucurbitacins displaying potentially important anti-inflammatory actions. The current study was designed to investigate the anti-ROS and -RNS effects of cucurbitacin L 2-O-β-glucoside (CLG) and the role of these signaling factors in the apoptogenic effects of CLG on human colon cancer cells (HT-29). This natural cucurbitacin was isolated purely from Citrullus lanatus var. citroides (Cucurbitaceae). The results revealed that CLG was cytotoxic to HT-29. CLG increased significantly (P