Chromosomal Composition of Micronuclei in Human Leukocytes Exposed to Mitomycin C

Micronuclei (MN) can be induced by different mutagenic substances. Even though this has been known for decades, it is still not clear which genetic content, especially which chromosomes, these MN are constituted of and if there are any influences on this content by the MN-inducing substance. Also, t...

Full description

Saved in:
Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 2012-04, Vol.60 (4), p.316-322
Main Authors: Hovhannisyan, Galina, Aroutiounian, Rouben, Liehr, Thomas
Format: Article
Language:English
Subjects:
Chromosomes, Human
Humans
In Situ Hybridization, Fluorescence
Leukocytes - drug effects
Leukocytes - ultrastructure
Micronucleus Tests
Mitomycin - toxicity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Micronuclei (MN) can be induced by different mutagenic substances. Even though this has been known for decades, it is still not clear which genetic content, especially which chromosomes, these MN are constituted of and if there are any influences on this content by the MN-inducing substance. Also, the interphase position, size, and gene density of a chromosome could influence its involvement in MN formation. To study some of these questions, fluorescence in situ hybridization using centromeric and whole-chromosome painting probes for chromosomes 3, 4, 6, 7, 9, 16, 17, 18, and X was applied in mitomycin C (MMC)–induced MN in human leukocytes. The obtained results showed that material from all studied chromosomes was present in MN. Also, there was no correlation between interphase position, size, and gene density of the studied chromosomes and their migration in MN. Interestingly, material derived from chromosomes 9 and 16 was overrepresented in MMC-induced MN. Finally, further studies using substances other than MMC are necessary to clarify if the MN-inducing mutagen has an influence on the chromosomal content of the MN.
ISSN:0022-1554
1551-5044
DOI:10.1369/0022155412436587