Antiviral activity and possible mechanisms of action of oligonucleotides-poly(L-lysine) conjugates targeted to vesicular stomatitis virus mRNA and genomic RNA

Synthetic oligonucleotides (oligomers) complementary to vesicular stomatitis virus (VSV) N protein mRNA have specific antiviral properties at concentrations lower than 1 microM when they are covalently linked to poly(L-lysine) (PLL). Since it is generally postulated that antisense oligomers act at t...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1989-11, Vol.17 (22), p.9341-9350
Main Authors: DEGOLS, G, LEONETTI, J.-P, GAGNOR, C, LEMAITRE, M, LEBLEU, B
Format: Article
Language:English
Subjects:
Animals
Antiviral Agents
Base Sequence
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Genes, Viral - drug effects
L Cells (Cell Line)
Mice
Miscellaneous
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Oligodeoxyribonucleotides - pharmacology
Polylysine
RNA - genetics
RNA, Antisense
RNA, Messenger - antagonists & inhibitors
RNA, Messenger - genetics
RNA, Viral - antagonists & inhibitors
RNA, Viral - genetics
Vesicular stomatitis Indiana virus - drug effects
Vesicular stomatitis Indiana virus - genetics
Vesicular stomatitis virus
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Synthetic oligonucleotides (oligomers) complementary to vesicular stomatitis virus (VSV) N protein mRNA have specific antiviral properties at concentrations lower than 1 microM when they are covalently linked to poly(L-lysine) (PLL). Since it is generally postulated that antisense oligomers act at the translational level, oligomers with potential targets on VSV viral mRNA and/or genomic RNA have been tested here. In vitro translation experiments in reticulocyte lysates, in vitro transcription experiments with permeabilized viruses, measurement of viral RNA transcription and accumulation in VSV infected cells, and antiviral experiments demonstrate in our model that antisense oligomers probably also act at other levels. Difficulties in the choice of the most effective antisense oligomer targets are also discussed.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/17.22.9341