Hexanucleotide motifs mediate recruitment of the RNA elimination machinery to silent meiotic genes

The selective elimination system blocks the accumulation of meiosis-specific mRNAs during the mitotic cell cycle in fission yeast. These mRNAs harbour a region, the determinant of selective removal (DSR), which is recognized by a YTH-family RNA-binding protein, Mmi1. Mmi1 directs target transcripts...

Full description

Saved in:
Bibliographic Details
Published in:Open biology 2012-03, Vol.2 (3), p.120014
Main Authors: Yamashita, Akira, Shichino, Yuichi, Tanaka, Hirotsugu, Hiriart, Edwige, Touat-Todeschini, Leila, Vavasseur, Aurélia, Ding, Da-Qiao, Hiraoka, Yasushi, Verdel, André, Yamamoto, Masayuki
Format: Article
Language:English
Subjects:
Base Sequence
Exosome
Exosomes - metabolism
Gene Silencing
Genes, Fungal
Genetics
Life Sciences
Meiosis
Meiosis - genetics
Mmi1
mRNA Cleavage and Polyadenylation Factors - genetics
mRNA Cleavage and Polyadenylation Factors - metabolism
Mrna Degradation
Mutagenesis
RNA, Fungal - chemistry
RNA, Fungal - genetics
RNA, Fungal - metabolism
RNA-binding protein
Schizosaccharomyces - cytology
Schizosaccharomyces - genetics
Schizosaccharomyces - metabolism
Schizosaccharomyces pombe Proteins - genetics
Schizosaccharomyces pombe Proteins - metabolism
Tandem Repeat Sequences
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The selective elimination system blocks the accumulation of meiosis-specific mRNAs during the mitotic cell cycle in fission yeast. These mRNAs harbour a region, the determinant of selective removal (DSR), which is recognized by a YTH-family RNA-binding protein, Mmi1. Mmi1 directs target transcripts to destruction in association with nuclear exosomes. Hence, the interaction between DSR and Mmi1 is crucial to discriminate mitosis from meiosis. Here, we show that Mmi1 interacts with repeats of the hexanucleotide U(U/C)AAAC that are enriched in the DSR. Disruption of this ‘DSR core motif’ in a target mRNA inhibits its elimination. Tandem repeats of the motif can function as an artificial DSR. Mmi1 binds to it in vitro. Thus, a core motif cluster is responsible for the DSR activity. Furthermore, certain variant hexanucleotide motifs can augment the function of the DSR core motif. Notably, meiRNA, which composes the nuclear Mei2 dot required to suppress Mmi1 activity during meiosis, carries numerous copies of the core/augmenting motifs on its tail and is indeed degraded by the Mmi1/exosome system, indicating its likely role as decoy bait for Mmi1.
ISSN:2046-2441
2046-2441
DOI:10.1098/rsob.120014