Loading…
Glypican-3 expression and its relationship with recurrence of HCC after liver transplantation
AIM： To investigate the diagnostic value of glypican-3 （GPC3） and its relationship with hepatocellular carci- noma （HCC） recurrence after liver transplantation. METHODS： HCC tissue samples （n = 31） obtained from patients who had undergone liver transplantation were analyzed. GPC3 mRNA and protein ex...
Saved in:
| Published in: | World journal of gastroenterology : WJG 2012-05, Vol.18 (19), p.2408-2414 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c422t-28c138fe01a259b86b21d6930ced7bba3bbce4b6bb0fb74594fc1208c9200b753 |
|---|---|
| cites | |
| container_end_page | 2414 |
| container_issue | 19 |
| container_start_page | 2408 |
| container_title | World journal of gastroenterology : WJG |
| container_volume | 18 |
| creator | Wang, Yu-Liang Zhu, Zhi-Jun Teng, Da-Hong Yao, Zhi Gao, Wei Shen, Zhong-Yang |
| description | AIM： To investigate the diagnostic value of glypican-3 （GPC3） and its relationship with hepatocellular carci- noma （HCC） recurrence after liver transplantation.
METHODS： HCC tissue samples （n = 31） obtained from patients who had undergone liver transplantation were analyzed. GPC3 mRNA and protein expression were analyzed by TaqMan real-time reverse transcription-polymerase chain reaction and immunohistochemistry. Correlation between the GPC3 expression and clinicopathological features was analyzed. The potential prognostic value of GPC3 was investigated by comparing recurrence-free survival between HCC patients with and without GPC3 expression.
RESULTS： Using a cutoff value of 3.5× 10^-2, 20 of 31 cancerous tissues had expression values of 〉 3.5× 10^2, whereas 3 of 31 adjacent non-neoplastic paren-chyma and 0 of 20 control liver tissues had expression values of 〉 3.5 × 10^-2 （P 〈 0.001）. GPC3 protein was immunoexpressed in 68% of cancerous tissues, but not in adjacent non-neoplastic parenchyma and control liver tissues. Vascular invasion was significantly related to GPC3 expression （P 〈 0.05）. Recurrence-free sur- vival was significantly longer for patients without GPC3 mRNA overexpression （〉 3.5 × 10^2） and those without vascular invasion （P 〈 0.05 for both）.
CONCLUSION： GPC3 expression may serve as a valuable diagnostic marker for HCC. GPC3 mRNA overexpression may be an adverse indicator for HCC patients after liver transplantation. |
| doi_str_mv | 10.3748/wjg.v18.i19.2408 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3353377</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>41967598</cqvip_id><sourcerecordid>1018369232</sourcerecordid><originalsourceid>FETCH-LOGICAL-c422t-28c138fe01a259b86b21d6930ced7bba3bbce4b6bb0fb74594fc1208c9200b753</originalsourceid><addsrcrecordid>eNpVkUFvGyEUhFHVKHbS3Huq6K2XdeDBLnCpVFlNUilSL8mxQoBZG2vNrmHtJP8-JHat9gLSY2YY-BD6TMmMCS6vn9bL2Z7KWaBqBpzID2gKQFUFkpOPaEoJEZViICboIuc1IcBYDedoAtDUnDM-RX9uu5chOBMrhv3zkHzOoY_YxAUOY8bJd2Ysg7wKA34K46pM3C4lH53HfYvv5nNs2tEn3IV9WcdkYh46E8d32yd01pou-6vjfokeb34-zO-q-9-3v-Y_7ivHAcZS11EmW0-ogVpZ2Vigi0Yx4vxCWGuYtc5z21hLWit4rXjrKBDpFBBiRc0u0fdD7rCzG79wPpYinR5S2Jj0onsT9P8nMaz0st_r8h-MCVECvh0DUr_d-TzqTcjOd-Ulvt9lTQmVrFHAoEjJQepSn3Py7ekaSvQbFV2o6EJFFyr6jUqxfPm33snwF0MRfD1mrvq43Ia4PGk4VY2olWSv5taXdQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1018369232</pqid></control><display><type>article</type><title>Glypican-3 expression and its relationship with recurrence of HCC after liver transplantation</title><source>PubMed Central</source><creator>Wang, Yu-Liang ; Zhu, Zhi-Jun ; Teng, Da-Hong ; Yao, Zhi ; Gao, Wei ; Shen, Zhong-Yang</creator><creatorcontrib>Wang, Yu-Liang ; Zhu, Zhi-Jun ; Teng, Da-Hong ; Yao, Zhi ; Gao, Wei ; Shen, Zhong-Yang</creatorcontrib><description>AIM： To investigate the diagnostic value of glypican-3 （GPC3） and its relationship with hepatocellular carci- noma （HCC） recurrence after liver transplantation.
METHODS： HCC tissue samples （n = 31） obtained from patients who had undergone liver transplantation were analyzed. GPC3 mRNA and protein expression were analyzed by TaqMan real-time reverse transcription-polymerase chain reaction and immunohistochemistry. Correlation between the GPC3 expression and clinicopathological features was analyzed. The potential prognostic value of GPC3 was investigated by comparing recurrence-free survival between HCC patients with and without GPC3 expression.
RESULTS： Using a cutoff value of 3.5× 10^-2, 20 of 31 cancerous tissues had expression values of 〉 3.5× 10^2, whereas 3 of 31 adjacent non-neoplastic paren-chyma and 0 of 20 control liver tissues had expression values of 〉 3.5 × 10^-2 （P 〈 0.001）. GPC3 protein was immunoexpressed in 68% of cancerous tissues, but not in adjacent non-neoplastic parenchyma and control liver tissues. Vascular invasion was significantly related to GPC3 expression （P 〈 0.05）. Recurrence-free sur- vival was significantly longer for patients without GPC3 mRNA overexpression （〉 3.5 × 10^2） and those without vascular invasion （P 〈 0.05 for both）.
CONCLUSION： GPC3 expression may serve as a valuable diagnostic marker for HCC. GPC3 mRNA overexpression may be an adverse indicator for HCC patients after liver transplantation.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v18.i19.2408</identifier><identifier>PMID: 22654434</identifier><language>eng</language><publisher>United States: Baishideng Publishing Group Co., Limited</publisher><subject>Adult ; Aged ; Biomarkers, Tumor ; Brief ; Carcinoma, Hepatocellular - genetics ; Carcinoma, Hepatocellular - metabolism ; Carcinoma, Hepatocellular - surgery ; Case-Control Studies ; Disease-Free Survival ; Female ; Gene Expression Regulation, Neoplastic ; Glypicans - genetics ; Glypicans - metabolism ; Humans ; Liver Neoplasms - genetics ; Liver Neoplasms - metabolism ; Liver Neoplasms - surgery ; Liver Transplantation ; Male ; Middle Aged ; mRNA表达 ; Neoplasm Recurrence, Local - genetics ; Neoplasm Recurrence, Local - metabolism ; Prognosis ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis ; TaqMan ; 复发 ; 相关性分析 ; 聚合酶链反应 ; 肝癌 ; 肝移植 ; 诊断价值</subject><ispartof>World journal of gastroenterology : WJG, 2012-05, Vol.18 (19), p.2408-2414</ispartof><rights>2012 Baishideng Publishing Group Co., Limited. All rights reserved. 2012</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-28c138fe01a259b86b21d6930ced7bba3bbce4b6bb0fb74594fc1208c9200b753</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84123X/84123X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353377/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353377/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22654434$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Yu-Liang</creatorcontrib><creatorcontrib>Zhu, Zhi-Jun</creatorcontrib><creatorcontrib>Teng, Da-Hong</creatorcontrib><creatorcontrib>Yao, Zhi</creatorcontrib><creatorcontrib>Gao, Wei</creatorcontrib><creatorcontrib>Shen, Zhong-Yang</creatorcontrib><title>Glypican-3 expression and its relationship with recurrence of HCC after liver transplantation</title><title>World journal of gastroenterology : WJG</title><addtitle>World Journal of Gastroenterology</addtitle><description>AIM： To investigate the diagnostic value of glypican-3 （GPC3） and its relationship with hepatocellular carci- noma （HCC） recurrence after liver transplantation.
METHODS： HCC tissue samples （n = 31） obtained from patients who had undergone liver transplantation were analyzed. GPC3 mRNA and protein expression were analyzed by TaqMan real-time reverse transcription-polymerase chain reaction and immunohistochemistry. Correlation between the GPC3 expression and clinicopathological features was analyzed. The potential prognostic value of GPC3 was investigated by comparing recurrence-free survival between HCC patients with and without GPC3 expression.
RESULTS： Using a cutoff value of 3.5× 10^-2, 20 of 31 cancerous tissues had expression values of 〉 3.5× 10^2, whereas 3 of 31 adjacent non-neoplastic paren-chyma and 0 of 20 control liver tissues had expression values of 〉 3.5 × 10^-2 （P 〈 0.001）. GPC3 protein was immunoexpressed in 68% of cancerous tissues, but not in adjacent non-neoplastic parenchyma and control liver tissues. Vascular invasion was significantly related to GPC3 expression （P 〈 0.05）. Recurrence-free sur- vival was significantly longer for patients without GPC3 mRNA overexpression （〉 3.5 × 10^2） and those without vascular invasion （P 〈 0.05 for both）.
CONCLUSION： GPC3 expression may serve as a valuable diagnostic marker for HCC. GPC3 mRNA overexpression may be an adverse indicator for HCC patients after liver transplantation.</description><subject>Adult</subject><subject>Aged</subject><subject>Biomarkers, Tumor</subject><subject>Brief</subject><subject>Carcinoma, Hepatocellular - genetics</subject><subject>Carcinoma, Hepatocellular - metabolism</subject><subject>Carcinoma, Hepatocellular - surgery</subject><subject>Case-Control Studies</subject><subject>Disease-Free Survival</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Glypicans - genetics</subject><subject>Glypicans - metabolism</subject><subject>Humans</subject><subject>Liver Neoplasms - genetics</subject><subject>Liver Neoplasms - metabolism</subject><subject>Liver Neoplasms - surgery</subject><subject>Liver Transplantation</subject><subject>Male</subject><subject>Middle Aged</subject><subject>mRNA表达</subject><subject>Neoplasm Recurrence, Local - genetics</subject><subject>Neoplasm Recurrence, Local - metabolism</subject><subject>Prognosis</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>TaqMan</subject><subject>复发</subject><subject>相关性分析</subject><subject>聚合酶链反应</subject><subject>肝癌</subject><subject>肝移植</subject><subject>诊断价值</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNpVkUFvGyEUhFHVKHbS3Huq6K2XdeDBLnCpVFlNUilSL8mxQoBZG2vNrmHtJP8-JHat9gLSY2YY-BD6TMmMCS6vn9bL2Z7KWaBqBpzID2gKQFUFkpOPaEoJEZViICboIuc1IcBYDedoAtDUnDM-RX9uu5chOBMrhv3zkHzOoY_YxAUOY8bJd2Ysg7wKA34K46pM3C4lH53HfYvv5nNs2tEn3IV9WcdkYh46E8d32yd01pou-6vjfokeb34-zO-q-9-3v-Y_7ivHAcZS11EmW0-ogVpZ2Vigi0Yx4vxCWGuYtc5z21hLWit4rXjrKBDpFBBiRc0u0fdD7rCzG79wPpYinR5S2Jj0onsT9P8nMaz0st_r8h-MCVECvh0DUr_d-TzqTcjOd-Ulvt9lTQmVrFHAoEjJQepSn3Py7ekaSvQbFV2o6EJFFyr6jUqxfPm33snwF0MRfD1mrvq43Ia4PGk4VY2olWSv5taXdQ</recordid><startdate>20120521</startdate><enddate>20120521</enddate><creator>Wang, Yu-Liang</creator><creator>Zhu, Zhi-Jun</creator><creator>Teng, Da-Hong</creator><creator>Yao, Zhi</creator><creator>Gao, Wei</creator><creator>Shen, Zhong-Yang</creator><general>Baishideng Publishing Group Co., Limited</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120521</creationdate><title>Glypican-3 expression and its relationship with recurrence of HCC after liver transplantation</title><author>Wang, Yu-Liang ; Zhu, Zhi-Jun ; Teng, Da-Hong ; Yao, Zhi ; Gao, Wei ; Shen, Zhong-Yang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-28c138fe01a259b86b21d6930ced7bba3bbce4b6bb0fb74594fc1208c9200b753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biomarkers, Tumor</topic><topic>Brief</topic><topic>Carcinoma, Hepatocellular - genetics</topic><topic>Carcinoma, Hepatocellular - metabolism</topic><topic>Carcinoma, Hepatocellular - surgery</topic><topic>Case-Control Studies</topic><topic>Disease-Free Survival</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Glypicans - genetics</topic><topic>Glypicans - metabolism</topic><topic>Humans</topic><topic>Liver Neoplasms - genetics</topic><topic>Liver Neoplasms - metabolism</topic><topic>Liver Neoplasms - surgery</topic><topic>Liver Transplantation</topic><topic>Male</topic><topic>Middle Aged</topic><topic>mRNA表达</topic><topic>Neoplasm Recurrence, Local - genetics</topic><topic>Neoplasm Recurrence, Local - metabolism</topic><topic>Prognosis</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><topic>TaqMan</topic><topic>复发</topic><topic>相关性分析</topic><topic>聚合酶链反应</topic><topic>肝癌</topic><topic>肝移植</topic><topic>诊断价值</topic><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yu-Liang</creatorcontrib><creatorcontrib>Zhu, Zhi-Jun</creatorcontrib><creatorcontrib>Teng, Da-Hong</creatorcontrib><creatorcontrib>Yao, Zhi</creatorcontrib><creatorcontrib>Gao, Wei</creatorcontrib><creatorcontrib>Shen, Zhong-Yang</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yu-Liang</au><au>Zhu, Zhi-Jun</au><au>Teng, Da-Hong</au><au>Yao, Zhi</au><au>Gao, Wei</au><au>Shen, Zhong-Yang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glypican-3 expression and its relationship with recurrence of HCC after liver transplantation</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World Journal of Gastroenterology</addtitle><date>2012-05-21</date><risdate>2012</risdate><volume>18</volume><issue>19</issue><spage>2408</spage><epage>2414</epage><pages>2408-2414</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>AIM： To investigate the diagnostic value of glypican-3 （GPC3） and its relationship with hepatocellular carci- noma （HCC） recurrence after liver transplantation.
METHODS： HCC tissue samples （n = 31） obtained from patients who had undergone liver transplantation were analyzed. GPC3 mRNA and protein expression were analyzed by TaqMan real-time reverse transcription-polymerase chain reaction and immunohistochemistry. Correlation between the GPC3 expression and clinicopathological features was analyzed. The potential prognostic value of GPC3 was investigated by comparing recurrence-free survival between HCC patients with and without GPC3 expression.
RESULTS： Using a cutoff value of 3.5× 10^-2, 20 of 31 cancerous tissues had expression values of 〉 3.5× 10^2, whereas 3 of 31 adjacent non-neoplastic paren-chyma and 0 of 20 control liver tissues had expression values of 〉 3.5 × 10^-2 （P 〈 0.001）. GPC3 protein was immunoexpressed in 68% of cancerous tissues, but not in adjacent non-neoplastic parenchyma and control liver tissues. Vascular invasion was significantly related to GPC3 expression （P 〈 0.05）. Recurrence-free sur- vival was significantly longer for patients without GPC3 mRNA overexpression （〉 3.5 × 10^2） and those without vascular invasion （P 〈 0.05 for both）.
CONCLUSION： GPC3 expression may serve as a valuable diagnostic marker for HCC. GPC3 mRNA overexpression may be an adverse indicator for HCC patients after liver transplantation.</abstract><cop>United States</cop><pub>Baishideng Publishing Group Co., Limited</pub><pmid>22654434</pmid><doi>10.3748/wjg.v18.i19.2408</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1007-9327 |
| ispartof | World journal of gastroenterology : WJG, 2012-05, Vol.18 (19), p.2408-2414 |
| issn | 1007-9327 2219-2840 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3353377 |
| source | PubMed Central |
| subjects | Adult Aged Biomarkers, Tumor Brief Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - metabolism Carcinoma, Hepatocellular - surgery Case-Control Studies Disease-Free Survival Female Gene Expression Regulation, Neoplastic Glypicans - genetics Glypicans - metabolism Humans Liver Neoplasms - genetics Liver Neoplasms - metabolism Liver Neoplasms - surgery Liver Transplantation Male Middle Aged mRNA表达 Neoplasm Recurrence, Local - genetics Neoplasm Recurrence, Local - metabolism Prognosis Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis TaqMan 复发 相关性分析 聚合酶链反应 肝癌 肝移植 诊断价值 |
| title | Glypican-3 expression and its relationship with recurrence of HCC after liver transplantation |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T22%3A50%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Glypican-3%20expression%20and%20its%20relationship%20with%20recurrence%20of%20HCC%20after%20liver%20transplantation&rft.jtitle=World%20journal%20of%20gastroenterology%20:%20WJG&rft.au=Wang,%20Yu-Liang&rft.date=2012-05-21&rft.volume=18&rft.issue=19&rft.spage=2408&rft.epage=2414&rft.pages=2408-2414&rft.issn=1007-9327&rft.eissn=2219-2840&rft_id=info:doi/10.3748/wjg.v18.i19.2408&rft_dat=%3Cproquest_pubme%3E1018369232%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c422t-28c138fe01a259b86b21d6930ced7bba3bbce4b6bb0fb74594fc1208c9200b753%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1018369232&rft_id=info:pmid/22654434&rft_cqvip_id=41967598&rfr_iscdi=true |