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Interpreting Quantitative Cytomegalovirus DNA Testing: Understanding the Laboratory Perspective

Cytomegalovirus (CMV) is an important cause of morbidity and mortality in transplant patients, and is typically monitored using laboratory-developed quantitative molecular assays. Clinicians who use quantitative CMV DNA testing should be aware of a number of aspects of testing that will aid in decis...

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Published in:	Clinical infectious diseases 2012-06, Vol.54 (12), p.1793-1797
Main Authors:	Kraft, Colleen S., Armstrong, Wendy S., Caliendo, Angela M.
Format:	Article
Language:	English
Subjects:	Biological and medical sciences Blood Blood - virology Blood plasma Cytomegalovirus Cytomegalovirus - genetics Cytomegalovirus - isolation & purification Cytomegalovirus infections Cytomegalovirus Infections - diagnosis Cytomegalovirus Infections - virology Data Interpretation, Statistical Decision making Deoxyribonucleic acid Disease risk DNA DNA, Viral - analysis DNA, Viral - isolation & purification Humans Infectious diseases Invited MEDICAL MICROBIOLOGY Medical sciences Mortality Relapse Specimens Transplantation Transplantation - adverse effects Transplants & implants Viral diseases Viral load Viral Load - methods Viruses
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description	Cytomegalovirus (CMV) is an important cause of morbidity and mortality in transplant patients, and is typically monitored using laboratory-developed quantitative molecular assays. Clinicians who use quantitative CMV DNA testing should be aware of a number of aspects of testing that will aid in decision making while managing CMV disease in their patients. These include (1) the specimen type used (whole blood or plasma), (2) the limit of detection and limit of quantification chosen by the clinical laboratory, (3) the linear range of the assay, (4) the reproducibility of the assay within the institution, and (5) the wide variability of viral load values among different assays. The biologic properties of CMV, including its variability within the host and of its half-life, are also important factors in the clinical testing for this virus.
doi_str_mv	10.1093/cid/cis212
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Clinicians who use quantitative CMV DNA testing should be aware of a number of aspects of testing that will aid in decision making while managing CMV disease in their patients. These include (1) the specimen type used (whole blood or plasma), (2) the limit of detection and limit of quantification chosen by the clinical laboratory, (3) the linear range of the assay, (4) the reproducibility of the assay within the institution, and (5) the wide variability of viral load values among different assays. The biologic properties of CMV, including its variability within the host and of its half-life, are also important factors in the clinical testing for this virus.</description><identifier>ISSN: 1058-4838</identifier><identifier>EISSN: 1537-6591</identifier><identifier>DOI: 10.1093/cid/cis212</identifier><identifier>PMID: 22412060</identifier><identifier>CODEN: CIDIEL</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Biological and medical sciences ; Blood ; Blood - virology ; Blood plasma ; Cytomegalovirus ; Cytomegalovirus - genetics ; Cytomegalovirus - isolation & purification ; Cytomegalovirus infections ; Cytomegalovirus Infections - diagnosis ; Cytomegalovirus Infections - virology ; Data Interpretation, Statistical ; Decision making ; Deoxyribonucleic acid ; Disease risk ; DNA ; DNA, Viral - analysis ; DNA, Viral - isolation & purification ; Humans ; Infectious diseases ; Invited ; MEDICAL MICROBIOLOGY ; Medical sciences ; Mortality ; Relapse ; Specimens ; Transplantation ; Transplantation - adverse effects ; Transplants & implants ; Viral diseases ; Viral load ; Viral Load - methods ; Viruses</subject><ispartof>Clinical infectious diseases, 2012-06, Vol.54 (12), p.1793-1797</ispartof><rights>Copyright © 2012 Oxford University Press on behalf of the Infectious Diseases Society of America</rights><rights>The Author 2012. 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Clinicians who use quantitative CMV DNA testing should be aware of a number of aspects of testing that will aid in decision making while managing CMV disease in their patients. These include (1) the specimen type used (whole blood or plasma), (2) the limit of detection and limit of quantification chosen by the clinical laboratory, (3) the linear range of the assay, (4) the reproducibility of the assay within the institution, and (5) the wide variability of viral load values among different assays. The biologic properties of CMV, including its variability within the host and of its half-life, are also important factors in the clinical testing for this virus.</description><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Blood - virology</subject><subject>Blood plasma</subject><subject>Cytomegalovirus</subject><subject>Cytomegalovirus - genetics</subject><subject>Cytomegalovirus - isolation & purification</subject><subject>Cytomegalovirus infections</subject><subject>Cytomegalovirus Infections - diagnosis</subject><subject>Cytomegalovirus Infections - virology</subject><subject>Data Interpretation, Statistical</subject><subject>Decision making</subject><subject>Deoxyribonucleic acid</subject><subject>Disease risk</subject><subject>DNA</subject><subject>DNA, Viral - analysis</subject><subject>DNA, Viral - isolation & purification</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Invited</subject><subject>MEDICAL MICROBIOLOGY</subject><subject>Medical sciences</subject><subject>Mortality</subject><subject>Relapse</subject><subject>Specimens</subject><subject>Transplantation</subject><subject>Transplantation - adverse effects</subject><subject>Transplants & implants</subject><subject>Viral diseases</subject><subject>Viral load</subject><subject>Viral Load - methods</subject><subject>Viruses</subject><issn>1058-4838</issn><issn>1537-6591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp9kcuLFDEQhxtR3HX14l1pEEGE0byT9iAs42th8AG751CdpGd76EnaJD0w_71pZtxRDx5CAvXVlyp-VfUUozcYNfSt6W05iWByrzrHnMqF4A2-X96IqwVTVJ1Vj1LaIISxQvxhdUYIwwQJdF7pK59dHKPLvV_XPybwuc-Q-52rl_sctm4NQ9j1cUr1h6-X9bVLM_iuvvHWxZTB27kv37p6BW2IkEPc199LaXRmtjyuHnQwJPfkeF9UN58-Xi-_LFbfPl8tL1cLw5TKC9JJ3JpGEWaspVg0CABk13FhlKO2QYhZjgi3DrfCydZK1dIG2lYwaIWw9KJ6f_COU7t11jifIwx6jP0W4l4H6PXfFd_f6nXYaUq5ZFIVwaujIIafU1lTb_tk3DCAd2FKGiPMJWdM0IK--AfdhCn6sl6hiJCMKjULXx8oE0NK0XV3w2Ck59x0yU0fcivw8z_Hv0N_B1WAl0cAkoGhi-BL64njjVCYyRMXpvH_Hz47cJtUIjt5KMGUUUx_ASTLudE</recordid><startdate>20120615</startdate><enddate>20120615</enddate><creator>Kraft, Colleen S.</creator><creator>Armstrong, Wendy S.</creator><creator>Caliendo, Angela M.</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T2</scope><scope>7T7</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120615</creationdate><title>Interpreting Quantitative Cytomegalovirus DNA Testing: Understanding the Laboratory Perspective</title><author>Kraft, Colleen S. ; 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subjects	Biological and medical sciences Blood Blood - virology Blood plasma Cytomegalovirus Cytomegalovirus - genetics Cytomegalovirus - isolation & purification Cytomegalovirus infections Cytomegalovirus Infections - diagnosis Cytomegalovirus Infections - virology Data Interpretation, Statistical Decision making Deoxyribonucleic acid Disease risk DNA DNA, Viral - analysis DNA, Viral - isolation & purification Humans Infectious diseases Invited MEDICAL MICROBIOLOGY Medical sciences Mortality Relapse Specimens Transplantation Transplantation - adverse effects Transplants & implants Viral diseases Viral load Viral Load - methods Viruses
title	Interpreting Quantitative Cytomegalovirus DNA Testing: Understanding the Laboratory Perspective
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