Two genes encode related cytoplasmic elongation factors 1α (EF-1α) in Drosophila melanogaster with continuous and stage specific expression

We have characterized two previously cloned genes, F1 and F2 (1) that code for elongation factor EF - 1 alpha of Drosophila melanogaster. Genomic Southern blot hybridization revealed that they are the only gene copies present. We isolated cDNA clones of both transcripts from embryonal and pupal stag...

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Bibliographic Details
Published in:Nucleic acids research 1988-04, Vol.16 (8), p.3175-3194
Main Authors: HOVEMANN, B, RICHTER, S, WALLDORF, U, CZIEPLUCH, C
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
DNA - genetics
Drosophila melanogaster
Drosophila melanogaster - genetics
Drosophila melanogaster - growth & development
Drosophilidae
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation
Genes
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Peptide Elongation Factor 1
Peptide Elongation Factors - genetics
Promoter Regions, Genetic
Sequence Homology, Nucleic Acid
Species Specificity
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Summary:We have characterized two previously cloned genes, F1 and F2 (1) that code for elongation factor EF - 1 alpha of Drosophila melanogaster. Genomic Southern blot hybridization revealed that they are the only gene copies present. We isolated cDNA clones of both transcripts from embryonal and pupal stage of development that cover the entire transcription unit. The 5' ends of both genes have been determined by primer extension and for F1 also by RNA sequencing. These start sites have been shown to be used consistently during development. Comparison of cDNA and genomic sequences revealed that EF - 1 alpha,F1 consists of two and EF - 1 alpha,F2 of five exons. The two described elongation factor genes exhibit several regions of strong sequence conservation when compared to five recently cloned eucaryotic elongation factors.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/16.8.3175