Specific binding of cruciform DNA structures by a protein from human extracts

A gel electrophoresis binding assay has been used to probe extracts from cultured human lymphoblasts for proteins that bind cruciform structures in duplex DNA. Proteins have been detected that form complexes with synthetic X- and Y-junctions. Several lines of evidence suggest that binding is specifi...

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Bibliographic Details
Published in:Nucleic acids research 1988-05, Vol.16 (9), p.3603-3616
Main Authors: ELBOROUGH, K. M, WEST, S. C
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Cell Line
DNA - metabolism
DNA Polymerase I - metabolism
DNA-Binding Proteins - isolation & purification
DNA-Binding Proteins - metabolism
Fundamental and applied biological sciences. Psychology
Humans
Interactions. Associations
Intermolecular phenomena
Molecular biophysics
Molecular Sequence Data
Nucleic Acid Conformation
Oligodeoxyribonucleotides - chemical synthesis
Oligodeoxyribonucleotides - metabolism
Protein Binding
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Summary:A gel electrophoresis binding assay has been used to probe extracts from cultured human lymphoblasts for proteins that bind cruciform structures in duplex DNA. Proteins have been detected that form complexes with synthetic X- and Y-junctions. Several lines of evidence suggest that binding is specific for DNA structure rather than sequence: (1) X- and Y-structures were bound whereas linear duplexes containing identical DNA sequences were not, (2) Binding occurred with equal efficiency to two X-junctions that were constructed from DNA strands of different sequence, (3) One X-junction successfully competed with another for binding whereas linear duplex DNA did not; and (4) protein-DNA complexes were observed at probe:non-specific competitor DNA ratios of 1:10,000.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/16.9.3603