Replication-induced stimulation of the major late promoter of adenovirus is correlated to the binding of a factor to sequences in the first intron

• Published in: Nucleic acids research 1988-05, Vol.16 (9), p.3771-3786
• Main Authors: JANSEN-DURR, P, BŒUF, H, KEDINGER, C
• Format: Article
• Language: English
• Subjects: Adenovirus; Adenoviruses, Human - genetics; Base Sequence; Biological and medical sciences; DNA Replication; DNA Restriction Enzymes; DNA, Viral - genetics; DNA-Binding Proteins - genetics; Fundamental and applied biological sciences. Psychology; Genes, Viral; HeLa Cells - metabolism; Humans; Introns; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Promoter Regions, Genetic; Transcription Factors - genetics; Transcription, Genetic; Transcription. Transcription factor. Splicing. Rna processing; Viral Proteins
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/16.9.3771

The sequence requirements for transcriptional stimulation of the adenovirus major late promoter (MLP) by the products of the early transcription unit Ela and by the replication of viral DNA were analyzed by in vitro transcription. Sequences upstream of +33 are involved in the moderate Ela-responsiveness of the MLP, while sequences between +33 and +131 are required for its major replication-induced transcriptional activation. Dnase I footprinting experiments delineate a sequence component, extending from +76 to +120, which binds protein(s) only in extracts of cells where viral DNA replication occurred. Taken together, these results suggest that the replication-dependent stimulation of the MLP is mediated by the increased binding of this protein(s).