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Characterization of immune modulating functions of γδ T cell subsets in a gnotobiotic pig model of human rotavirus infection

Abstract We characterized immune modulating functions of porcine γδ T cell subsets in rotavirus infection using a gnotobiotic pig model of human rotavirus infection and sort-purified lymphocyte autologous co-cultures. We demonstrated that CD2+CD8− and CD2−CD8− γδ T cells have mainly pro-inflammatory...

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Published in:Comparative immunology, microbiology and infectious diseases microbiology and infectious diseases, 2012-07, Vol.35 (4), p.289-301
Main Authors: Wen, Ke, Bui, Tammy, Li, Guohua, Liu, Fangning, Li, Yanru, Kocher, Jacob, Yuan, Lijuan
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container_title Comparative immunology, microbiology and infectious diseases
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creator Wen, Ke
Bui, Tammy
Li, Guohua
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Li, Yanru
Kocher, Jacob
Yuan, Lijuan
description Abstract We characterized immune modulating functions of porcine γδ T cell subsets in rotavirus infection using a gnotobiotic pig model of human rotavirus infection and sort-purified lymphocyte autologous co-cultures. We demonstrated that CD2+CD8− and CD2−CD8− γδ T cells have mainly pro-inflammatory function as evident by directly secreting IFN-γ or promoting CD4+ αβ T cell proliferation and IFN-γ production, whereas CD2+CD8+ γδ T cells mainly exert regulatory T cell function by expressing FoxP3, secreting IL-10 and TGF-β or increasing IL-10 and TGF-β production by CD4+ αβ T cells. γδ T cells responded to rotavirus infection by increasing TLR2, TLR3, TLR9 expression and IFN-γ and/or TGF-β production. The CD8− subsets likely differentiate into CD8+ subset by acquiring CD8 expression, explaining in part the apparently dual functions of CD2+CD8+ and CD2+CD8− subsets. Thus, both CD8+ and CD8− γδ T cell subsets can contribute to anti-rotavirus immunity and to the maintenance and restoration of intestinal and systemic homeostasis.
doi_str_mv 10.1016/j.cimid.2012.01.010
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subjects Allergy and Immunology
Animals
Biological and medical sciences
CD2 Antigens - metabolism
CD4-Positive T-Lymphocytes - immunology
CD4-Positive T-Lymphocytes - pathology
CD8-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - pathology
Coculture Techniques
Cytokines
Disease Models, Animal
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Gammadelta (γδ) T cell subset
Gene Expression
Germ-Free Life - immunology
Gnotobiotic pigs
Humans
Infectious Disease
Interferon-gamma - biosynthesis
Interferon-gamma - immunology
Interleukin-10 - biosynthesis
Interleukin-10 - immunology
Microbiology
Miscellaneous
Receptors, Antigen, T-Cell, gamma-delta - metabolism
Rotavirus
Rotavirus Infections - immunology
Rotavirus Infections - pathology
Rotavirus Infections - virology
Swine
T cell proliferation
T-Lymphocyte Subsets - immunology
T-Lymphocyte Subsets - pathology
Toll-like receptors
Toll-Like Receptors - genetics
Toll-Like Receptors - immunology
Transforming Growth Factor beta - biosynthesis
Transforming Growth Factor beta - immunology
Virology
title Characterization of immune modulating functions of γδ T cell subsets in a gnotobiotic pig model of human rotavirus infection
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