PFE0565w, a Plasmodium falciparum Protein Expressed in Salivary Gland Sporozoites

Because malaria is still a significant problem worldwide, additional control methods need to be developed. The Plasmodium sporozoite is a good target for control measures because it displays dual infectivity for both mosquito and vertebrate host tissues. The Plasmodium falciparum gene, PFE0565w, was...

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Bibliographic Details
Published in:The American journal of tropical medicine and hygiene 2012-06, Vol.86 (6), p.943-954
Main Authors: SCHLARMAN, Maggie S, ROBERTS, Renee N, KARIUKI, Michael M, LACRUE, Alexis N, RUGUANG OU, BEERNTSEN, Brenda T
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Erythrocytes - parasitology
Humans
Infectious diseases
Life Cycle Stages
Male
Medical sciences
Membrane Proteins - genetics
Membrane Proteins - metabolism
Microscopy, Confocal
Plasmodium falciparum
Plasmodium falciparum - genetics
Plasmodium falciparum - metabolism
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Salivary Glands - parasitology
Selection, Genetic
Sequence Analysis, DNA
Sporozoites - metabolism
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Summary:Because malaria is still a significant problem worldwide, additional control methods need to be developed. The Plasmodium sporozoite is a good target for control measures because it displays dual infectivity for both mosquito and vertebrate host tissues. The Plasmodium falciparum gene, PFE0565w, was chosen as a candidate for study based on data from PlasmoDB, the Plasmodium database, indicating that it is expressed both at the transcriptional and protein levels in sporozoites, likely encodes a putative surface protein, and may have a potential role in the invasion of host tissues. Additional sequence analysis shows that the PFE0565w protein has orthologs in other Plasmodium species, but none outside of the genus Plasmodium. PFE0565w expresses transcript during both the sporozoite and erythrocytic stages of the parasite life cycle, where an alternative transcript was discovered during the erythrocytic stages. Data show that transcript is not present during axenic exoerythrocytic stages. Despite transcript being present in several life cycle stages, the PFE0565w protein is present only during the salivary gland sporozoite stage. Because the PFE0565w protein is present in salivary gland sporozoites, it could be a novel candidate for a pre-erythrocytic stage vaccine.
ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.2012.11-0797