Polymerase chain reaction automated at low cost

The recent report in this journal of a simple, low-coast "DNA amplifier" has prompted the authors to describe the authors' experiences with a different mechanical apparatus that has been used successfully for several months in their laboratory. The authors have modified a Histokinette...

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Bibliographic Details
Published in:Nucleic acids research 1988-06, Vol.16 (12), p.5687-5688
Main Authors: FOULKES, N. S, PANDOLFI DE RINALDIS, P. P, MACDONNELL, J, CROSS, N. C. P, LUZZATTO, L
Format: Article
Language:English
Subjects:
Autoanalysis - instrumentation
Autoanalysis - methods
Biological and medical sciences
Deoxyribonucleases, Type II Site-Specific
Diverse techniques
DNA Restriction Enzymes - metabolism
DNA-directed DNA polymerase
Enzyme Stability
Fundamental and applied biological sciences. Psychology
Genes
Glucosephosphate Dehydrogenase - genetics
Humans
Molecular and cellular biology
polymerase chain reaction
Temperature
Thermus aquaticus
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Summary:The recent report in this journal of a simple, low-coast "DNA amplifier" has prompted the authors to describe the authors' experiences with a different mechanical apparatus that has been used successfully for several months in their laboratory. The authors have modified a Histokinette, a conventional tissue embedding instrument which has been employed in histopathology departments for the some thirty years, for the automation of PCR. A two tier, circular steel rack that can accommodate thirty six 0.5 ml microfuge tubes, was constructed and suspended from the mechanical arm. During one cycle, the arm pivot about the central axis and sequentially dips the rack into the three thermostatically controlled water baths. The tubes are completely submerged in each of the water baths; this reduces condensation and obviates the need for mineral oil. The process is repeated automatically for the required number of cycles.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/16.12.5687