Dynamic tissue analysis using time- and wavelength-resolved fluorescence spectroscopy for atherosclerosis diagnosis

Simultaneous time- and wavelength-resolved fluorescence spectroscopy (STWRFS) was developed and tested for the dynamic characterization of atherosclerotic tissue ex vivo and arterial vessels in vivo. Autofluorescence, induced by a 337 nm, 700 ps pulsed laser, was split to three wavelength sub-bands...

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Bibliographic Details
Published in:Optics express 2011-02, Vol.19 (5), p.3890-3901
Main Authors: Sun, Yinghua, Sun, Yang, Stephens, Douglas, Xie, Hongtao, Phipps, Jennifer, Saroufeem, Ramez, Southard, Jeffrey, Elson, Daniel S, Marcu, Laura
Format: Article
Language:English
Subjects:
Animals
Atherosclerosis - diagnosis
Equipment Design
Equipment Failure Analysis
Reproducibility of Results
Sensitivity and Specificity
Signal Processing, Computer-Assisted - instrumentation
Spectrometry, Fluorescence - instrumentation
Swine
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Summary:Simultaneous time- and wavelength-resolved fluorescence spectroscopy (STWRFS) was developed and tested for the dynamic characterization of atherosclerotic tissue ex vivo and arterial vessels in vivo. Autofluorescence, induced by a 337 nm, 700 ps pulsed laser, was split to three wavelength sub-bands using dichroic filters, with each sub-band coupled into a different length of optical fiber for temporal separation. STWRFS allows for fast recording/analysis (few microseconds) of time-resolved fluorescence emission in these sub-bands and rapid scanning. Distinct compositions of excised human atherosclerotic aorta were clearly discriminated over scanning lengths of several centimeters based on fluorescence lifetime and the intensity ratio between 390 and 452 nm. Operation of STWRFS blood flow was further validated in pig femoral arteries in vivo using a single-fiber probe integrated with an ultrasound imaging catheter. Current results demonstrate the potential of STWRFS as a tool for real-time optical characterization of arterial tissue composition and for atherosclerosis research and diagnosis.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.19.003890