A physical map of the genome of Mycoplasma mycoides subspecies mycoides Y with some functional loci

A physical map is presented for the 1200 kb genome of Mycoplasma mycoides subsp. mycoides Y, locating 32 cleavage sites for 8 restriction endonucleases. The large restriction fragments involved were separated and sized by pulsed-field agarose gel electrophoresis. Their locations on the map were dete...

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Bibliographic Details
Published in:Nucleic acids research 1988-07, Vol.16 (13), p.6027-6039
Main Authors: PYLE, L. E, FINCH, L. R
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Chromosome Mapping
DNA Replication
DNA Restriction Enzymes - metabolism
DNA, Fungal - analysis
Electrophoresis, Agar Gel
Fundamental and applied biological sciences. Psychology
Genetics
Microbiology
Mycoplasma - genetics
Mycoplasma mycoides
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Summary:A physical map is presented for the 1200 kb genome of Mycoplasma mycoides subsp. mycoides Y, locating 32 cleavage sites for 8 restriction endonucleases. The large restriction fragments involved were separated and sized by pulsed-field agarose gel electrophoresis. Their locations on the map were determined by probing Southern blots of digests with individual fragments isolated from other digests and by correlating the products of double and triple digestions. Loci for 2 ribosomal RNA operons and 2 tRNA operons have been determined by probing with cloned genes and the broad regions of the replication origin and terminus have also been outlined by in vivo labelling studies.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/16.13.6027