Characterisation of BciBII, an isoschizomer of BstNI from a strain of Bacillus circulans B

BciBII, an isoschizomer of BstNI has been isolated from Bacillus circulans B. The enzyme was purified by precipitation with polyethylenimine, ammonium sulfate and subsequent column chromatography on DEAE-sepharose, Blue sepharose and phosphocellulose. Restriction endonuclease BciBII showed maximal a...

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Bibliographic Details
Published in:Nucleic acids research 1992-06, Vol.20 (11), p.2896-2896
Main Authors: ELDAROV, M. A, KARPICHEV, I. V, SAMKO, O. T, ANIKEITCHEVA, N. V, KALUGIN, A. A, KHOROSHOUTINA, E. B, SOKOLOV, N. N
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Bacillus - enzymology
Bacillus circulans
Biological and medical sciences
Deoxyribonucleases, Type II Site-Specific - isolation & purification
Deoxyribonucleases, Type II Site-Specific - metabolism
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Hydrolases
Substrate Specificity
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Summary:BciBII, an isoschizomer of BstNI has been isolated from Bacillus circulans B. The enzyme was purified by precipitation with polyethylenimine, ammonium sulfate and subsequent column chromatography on DEAE-sepharose, Blue sepharose and phosphocellulose. Restriction endonuclease BciBII showed maximal activity at 37 degree C, pH between 7.4-7.8, MgCl sub(2) concentration in the range of 8-12 mM and at high ionic strength.
ISSN:0305-1048
1362-4962