Detection of Bioactive Exometabolites Produced by the Filamentous Marine Cyanobacterium Geitlerinema sp

Marine cyanobacteria are noted for their ability to excrete metabolites with biotic properties. This paper focuses on such exometabolites obtained from the culture of the marine filamentous cyanobacterium Geitlerinema sp. strain, their purification and subsequent analyses. By this means the recoveri...

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Bibliographic Details
Published in:Marine biotechnology (New York, N.Y.) N.Y.), 2012-08, Vol.14 (4), p.436-445
Main Authors: Caicedo, Nelson H, Kumirska, Jolanta, Neumann, Jennifer, Stolte, Stefan, Thöming, Jorg
Format: Article
Language:English
Subjects:
Analysis
Antibiotics
Antimicrobial agents
Bacteria
Bioactive compounds
bioactive properties
Biological Products - analysis
Biological Products - chemistry
Biological Products - metabolism
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Biphenyl Compounds - analysis
Biphenyl Compounds - chemistry
Biphenyl Compounds - metabolism
Chemical analysis
Culture media
Cyanobacteria
Cyanobacteria - classification
Cyanobacteria - metabolism
Cyanobacterium
Engineering
Freshwater & Marine Ecology
Gas chromatography
Geitlerinema
Harmine - analogs & derivatives
isopropyl alcohol
Life Sciences
Marine
Metabolites
Microbiology
Microorganisms
Natural products
Oceans and Seas
Original
Original Article
Scientific imaging
Species Specificity
Studies
Water Microbiology
Zoology
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Summary:Marine cyanobacteria are noted for their ability to excrete metabolites with biotic properties. This paper focuses on such exometabolites obtained from the culture of the marine filamentous cyanobacterium Geitlerinema sp. strain, their purification and subsequent analyses. By this means the recoveries of the active compounds, a prerequisite for properly determining their concentration, are quantified here for the first time. We demonstrate a new procedure using Amberlite XAD-1180 resin in combination with the eluent isopropanol for extraction of the culture media and gas chromatography as simplified chemical analysis. This procedure reduced necessary bacteria cultivation time (from 150 to 21 days) at low volumes of culture media (300 mL) required for identification of two selected bioactive compounds: 4,4′-dihydroxybiphenyl and harmane.
ISSN:1436-2228
1436-2236
DOI:10.1007/s10126-011-9424-1