Properties of mechano-transduction via simulated microgravity and its effects on intracellular trafficking of VEGFR's

• Published in: Oncotarget 2012-04, Vol.3 (4), p.426-434
• Main Authors: Puca, Andrew, Russo, Giuseppe, Giordano, Antonio
• Format: Article
• Language: English
• Subjects: Adult; Antigens, CD34 - metabolism; Bone Marrow Cells - cytology; Bone Marrow Cells - metabolism; Cell Proliferation; Cell Survival; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - metabolism; Humans; Intracellular Space; Jurkat Cells; Leukemia - metabolism; Leukemia - pathology; Mechanotransduction, Cellular; Protein Transport; Receptors, Vascular Endothelial Growth Factor - metabolism; Research Papers; Weightlessness Simulation
• ISSN: 1949-2553; 1949-2553
• DOI: 10.18632/oncotarget.472

This study emphasizes the dynamical properties of mechanical loading via simulated microgravity, its effect on acute myeloid leukemia proliferation and hematopoietic stem cell (HSPC) growth and its implications in the area of tissue regeneration. Data presented illustrates that mechanical transduction changes the expression of humoral factors by facilitating paracrine/autocrine signalling, therefore modulating intracellular trafficking of tyrosine kinase receptors. Understanding mechano-transduction in the context of cell and tissue morphogenesis is the major focus of this study. The effects of external physiological stresses, such as blood flow, on several cellular subtypes seem to produce very intricate cellular responses. It is well accepted that mechanical loading plays an intrinsic and extrinsic influence on cell survival. This study shows how microgravity effects hematopoietic stem cells, and human leukemic cell proliferation and expression of its receptors that control cell survival, such as the tyrosine kinase vascular endothelial growth factor receptor-1, receptor-2 and receptor-3.