In vivo imaging of unstained tissues using a compact and flexible multiphoton microendoscope

We use a compact and flexible multiphoton microendoscope (MPME) to acquire in vivo images of unstained liver, kidney, and colon from an anesthetized rat. The device delivers femtosecond pulsed 800 nm light from the core of a raster-scanned dual-clad fiber (DCF), which is focused by a miniaturized gr...

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Bibliographic Details
Published in:Journal of biomedical optics 2012-04, Vol.17 (4), p.040505-040505
Main Authors: Brown, Christopher M, Rivera, David R, Pavlova, Ina, Ouzounov, Dimitre G, Williams, Wendy O, Mohanan, Sunish, Webb, Watt W, Xu, Chris
Format: Article
Language:English
Subjects:
Animals
Biocompatibility
Biomedical materials
Colon
Colon - chemistry
Devices
Endoscopes
Fiber Optic Technology - instrumentation
Image acquisition
In vivo testing
In vivo tests
JBO Letters
Kidney - chemistry
Liver - chemistry
Male
Microscopy, Fluorescence, Multiphoton - instrumentation
Microscopy, Fluorescence, Multiphoton - methods
Microtechnology - instrumentation
Rats
Rats, Sprague-Dawley
Surgical implants
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Summary:We use a compact and flexible multiphoton microendoscope (MPME) to acquire in vivo images of unstained liver, kidney, and colon from an anesthetized rat. The device delivers femtosecond pulsed 800 nm light from the core of a raster-scanned dual-clad fiber (DCF), which is focused by a miniaturized gradient-index lens assembly into tissue. Intrinsic fluorescence and second-harmonic generation signal from the tissue is epi-collected through the core and inner clad of the same DCF. The MPME has a rigid distal tip of 3 mm in outer diameter and 4 cm in length. The image field-of-view measures 115 μm by 115 μm and was acquired at 4.1 frames/s with 75 mW illumination power at the sample. Organs were imaged after anesthetizing Sprague-Dawley rats with isofluorane gas, accessing tissues via a ventral-midline abdominal incision, and isolating the organs with tongue depressors. In vivo multiphoton images acquired from liver, kidney, and colon using this device show features similar to that of conventional histology slides, without motion artifact, in ~75% of imaged frames. To the best of our knowledge, this is the first demonstration of multiphoton imaging of unstained tissue from a live subject using a compact and flexible MPME device.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.jbo.17.4.040505