Protective Properties of Laggera alata Extract and its Principle Components Against d-Galactosamine-Injured Hepatocytes

• Published in: Scientia pharmaceutica 2012-04, Vol.80 (2), p.447-456
• Main Authors: Wu, Yi-Hang, Hao, Bing-Jie, Shen, E, Meng, Qing-Li, Hu, Ming-Hui, Zhao, Yu
• Format: Article
• Language: English
• ISSN: 0036-8709; 2218-0532
• DOI: 10.3797/scipharm.1108-16

Laggera alata extract (LAE) was quantitatively analyzed, and its principle components isochlorogenic acids were isolated and authenticated. Protective properties of LAE were studied using a d-galactosamine (d-GalN)-induced injury model in neonatal rat hepatocytes and a d-GalN-induced acute liver damage model in mice. Meanwhile, the effect of isochlorogenic acids derived from LAE on d-GalN-induced hepatocyte injury were also measured in vitro. LAE at concentrations of 10-100 μg/ml significantly reduced cellular leakage of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and improved cell viability. The isochlorogenic acids (4,5-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid and 3,4-O-dicaffeoylquinic acid) at concentrations of 1-100 μg/ml also remarkably improved viability of hepatocytes. The oral treatment of LAE at doses of 50, 100 and 200 mg/kg markedly reduced the serum AST and ALT activity of mice and resulted in significant recovery of hepatocytes in liver sections.