Suppressive effects of natural reduced waters on alloxan-induced apoptosis and type 1 diabetes mellitus

• Published in: Cytotechnology (Dordrecht) 2012-05, Vol.64 (3), p.281-297
• Main Authors: Li, Yuping, Hamasaki, Takeki, Teruya, Kiichiro, Nakamichi, Noboru, Gadek, Zbigniew, Kashiwagi, Taichi, Yan, Hanxu, Kinjo, Tomoya, Komatsu, Takaaki, Ishii, Yoshitoki, Shirahata, Sanetaka
• Format: Article
• Language: English
• Subjects: Apoptosis; Beta cells; Biochemistry; Biological and medical sciences; Biomedicine; Biotechnology; Blood levels; Catalase; Cell cycle; Chemistry; Chemistry and Materials Science; Cytotoxicity; Diabetes; Diabetes mellitus (insulin dependent); DNA fragmentation; Electrodes; Fundamental and applied biological sciences. Psychology; G1 phase; Insulin; JAACT Special Issue; Mineral water; Penicillin; Reactive oxygen species
• ISSN: 0920-9069; 1573-0778
• DOI: 10.1007/s10616-011-9414-1

Insulin-producing cells express limited activities of anti-oxidative enzymes. Therefore, reactive oxygen species (ROS) produced in these cells play a crucial role in cytotoxic effects. Furthermore, diabetes mellitus (DM) development is closely linked to higher ROS levels in insulin-producing cells. Hita Tenryosui Water ® (Hita T. W., Hita, Japan) and Nordenau water (Nord. W., Nordenau, Germany), referred to as natural reduced waters (NRWs), scavenge ROS in cultured cells, and therefore, might be a possibility as an alternative to conventional pharmacological agents against DM. Therefore, this study aimed to investigate the role of NRWs in alloxan (ALX)-induced β-cell apoptosis as well as in ALX-induced diabetic mice. NRWs equally suppressed DNA fragmentation levels. Hita T. W. and Nord. W. ameliorated ALX-induced sub-G 1 phase production from approximately 40% of control levels to 8.5 and 11.8%, respectively. NRWs restored serum insulin levels ( p