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Dissecting Arabidopsis Gβ Signal Transduction on the Protein Surface
The heterotrimeric G-protein complex provides signal amplification and target specificity. The Arabidopsis (Arabidopsis thaliana) Gβ-subunit of this complex (AGB1) interacts with and modulates the activity of target cytoplasmic proteins. This specificity resides in the structure of the interface bet...
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| Published in: | Plant physiology (Bethesda) 2012-07, Vol.159 (3), p.975-983 |
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| Main Authors: | , , , , , , , , , , , |
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| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c439t-df576c4917e605dfc84b5d1f10ab4957727d57c8b17cd7ef37bbab7978eabb273 |
| container_end_page | 983 |
| container_issue | 3 |
| container_start_page | 975 |
| container_title | Plant physiology (Bethesda) |
| container_volume | 159 |
| creator | Jiang, Kun Frick-Cheng, Arwen Trusov, Yuri Delgado-Cerezo, Magdalena Rosenthal, David M. Lorek, Justine Panstruga, Ralph Booker, Fitzgerald L. Botella, José Ramón Molina, Antonio Ort, Donald R. Jones, Alan M. |
| description | The heterotrimeric G-protein complex provides signal amplification and target specificity. The Arabidopsis (Arabidopsis thaliana) Gβ-subunit of this complex (AGB1) interacts with and modulates the activity of target cytoplasmic proteins. This specificity resides in the structure of the interface between AGB1 and its targets. Important surface residues of AGB1, which were deduced from a comparative evolutionary approach, were mutated to dissect AGB1-dependent physiological functions. Analysis of the capacity of these mutants to complement well-established phenotypes of Gβ-null mutants revealed AGB1 residues critical for specific AGB1-mediated biological processes, including growth architecture, pathogen resistance, stomata-mediated leaf-air gas exchange, and possibly photosynthesis. These findings provide promising new avenues to direct the finely tuned engineering of crop yield and traits. |
| doi_str_mv | 10.1104/pp.112.196337 |
| format | article |
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The Arabidopsis (Arabidopsis thaliana) Gβ-subunit of this complex (AGB1) interacts with and modulates the activity of target cytoplasmic proteins. This specificity resides in the structure of the interface between AGB1 and its targets. Important surface residues of AGB1, which were deduced from a comparative evolutionary approach, were mutated to dissect AGB1-dependent physiological functions. Analysis of the capacity of these mutants to complement well-established phenotypes of Gβ-null mutants revealed AGB1 residues critical for specific AGB1-mediated biological processes, including growth architecture, pathogen resistance, stomata-mediated leaf-air gas exchange, and possibly photosynthesis. These findings provide promising new avenues to direct the finely tuned engineering of crop yield and traits.</description><identifier>ISSN: 0032-0889</identifier><identifier>ISSN: 1532-2548</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.112.196337</identifier><identifier>PMID: 22570469</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Biologists</publisher><subject>Abscisic Acid - pharmacology ; Agriculture ; Arabidopsis - genetics ; Arabidopsis - growth & development ; Arabidopsis - metabolism ; Arabidopsis - microbiology ; Arabidopsis Proteins - metabolism ; Biological and medical sciences ; CELL BIOLOGY AND SIGNAL TRANSDUCTION ; Flagellin - pharmacology ; Fundamental and applied biological sciences. Psychology ; Fungi ; Glucose - pharmacology ; GTP-Binding Protein beta Subunits - metabolism ; Hypocotyls ; Leaves ; Models, Molecular ; Mutagenesis, Site-Directed ; Mutant Proteins - metabolism ; Mutation - genetics ; Phenotype ; Phenotypes ; Plant cells ; Plant physiology and development ; Plant roots ; Plants ; Plants, Genetically Modified ; Protein Folding - drug effects ; Reactive Oxygen Species - metabolism ; Rice ; Seedlings ; Signal Transduction - drug effects ; Surface Properties - drug effects</subject><ispartof>Plant physiology (Bethesda), 2012-07, Vol.159 (3), p.975-983</ispartof><rights>2012 American Society of Plant Biologists</rights><rights>2015 INIST-CNRS</rights><rights>2012 American Society of Plant Biologists. All rights reserved. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-df576c4917e605dfc84b5d1f10ab4957727d57c8b17cd7ef37bbab7978eabb273</citedby><cites>FETCH-LOGICAL-c439t-df576c4917e605dfc84b5d1f10ab4957727d57c8b17cd7ef37bbab7978eabb273</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/41549916$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/41549916$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,885,27923,27924,58237,58470</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26144801$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22570469$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, Kun</creatorcontrib><creatorcontrib>Frick-Cheng, Arwen</creatorcontrib><creatorcontrib>Trusov, Yuri</creatorcontrib><creatorcontrib>Delgado-Cerezo, Magdalena</creatorcontrib><creatorcontrib>Rosenthal, David M.</creatorcontrib><creatorcontrib>Lorek, Justine</creatorcontrib><creatorcontrib>Panstruga, Ralph</creatorcontrib><creatorcontrib>Booker, Fitzgerald L.</creatorcontrib><creatorcontrib>Botella, José Ramón</creatorcontrib><creatorcontrib>Molina, Antonio</creatorcontrib><creatorcontrib>Ort, Donald R.</creatorcontrib><creatorcontrib>Jones, Alan M.</creatorcontrib><title>Dissecting Arabidopsis Gβ Signal Transduction on the Protein Surface</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>The heterotrimeric G-protein complex provides signal amplification and target specificity. The Arabidopsis (Arabidopsis thaliana) Gβ-subunit of this complex (AGB1) interacts with and modulates the activity of target cytoplasmic proteins. This specificity resides in the structure of the interface between AGB1 and its targets. Important surface residues of AGB1, which were deduced from a comparative evolutionary approach, were mutated to dissect AGB1-dependent physiological functions. Analysis of the capacity of these mutants to complement well-established phenotypes of Gβ-null mutants revealed AGB1 residues critical for specific AGB1-mediated biological processes, including growth architecture, pathogen resistance, stomata-mediated leaf-air gas exchange, and possibly photosynthesis. These findings provide promising new avenues to direct the finely tuned engineering of crop yield and traits.</description><subject>Abscisic Acid - pharmacology</subject><subject>Agriculture</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - growth & development</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis - microbiology</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Biological and medical sciences</subject><subject>CELL BIOLOGY AND SIGNAL TRANSDUCTION</subject><subject>Flagellin - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungi</subject><subject>Glucose - pharmacology</subject><subject>GTP-Binding Protein beta Subunits - metabolism</subject><subject>Hypocotyls</subject><subject>Leaves</subject><subject>Models, Molecular</subject><subject>Mutagenesis, Site-Directed</subject><subject>Mutant Proteins - metabolism</subject><subject>Mutation - genetics</subject><subject>Phenotype</subject><subject>Phenotypes</subject><subject>Plant cells</subject><subject>Plant physiology and development</subject><subject>Plant roots</subject><subject>Plants</subject><subject>Plants, Genetically Modified</subject><subject>Protein Folding - drug effects</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Rice</subject><subject>Seedlings</subject><subject>Signal Transduction - drug effects</subject><subject>Surface Properties - drug effects</subject><issn>0032-0889</issn><issn>1532-2548</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNpVkM1KAzEUhYMotlaXLpXZuJyaTJLJZCOUWqsgKLSuQ_6mTWlnhmQq-Fo-iM9kytSqcOFcuN89Bw4AlwgOEYLktmmiZkPEc4zZEegjirM0o6Q4Bn0I4w6LgvfAWQgrCCHCiJyCXpZRBknO-2By70KwunXVIhl5qZypm-BCMv36TGZuUcl1MveyCmYbmbpK4rRLm7z6urWuSmZbX0ptz8FJKdfBXux1AN4eJvPxY_r8Mn0aj55TTTBvU1NSlmvCEbM5pKbUBVHUoBJBqQinjGXMUKYLhZg2zJaYKSUV46ywUqmM4QG463ybrdpYo23VerkWjXcb6T9ELZ34f6ncUizqd4FxEd1RNEg7A-3rELwtD78Iil2fommiZqLrM_LXfwMP9E-BEbjZAzJouS5jV9qFXy5HhBRwF3zVcavQ1v5wJ4gSzlGOvwF2FIm2</recordid><startdate>20120701</startdate><enddate>20120701</enddate><creator>Jiang, Kun</creator><creator>Frick-Cheng, Arwen</creator><creator>Trusov, Yuri</creator><creator>Delgado-Cerezo, Magdalena</creator><creator>Rosenthal, David M.</creator><creator>Lorek, Justine</creator><creator>Panstruga, Ralph</creator><creator>Booker, Fitzgerald L.</creator><creator>Botella, José Ramón</creator><creator>Molina, Antonio</creator><creator>Ort, Donald R.</creator><creator>Jones, Alan M.</creator><general>American Society of Plant Biologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20120701</creationdate><title>Dissecting Arabidopsis Gβ Signal Transduction on the Protein Surface</title><author>Jiang, Kun ; Frick-Cheng, Arwen ; Trusov, Yuri ; Delgado-Cerezo, Magdalena ; Rosenthal, David M. ; Lorek, Justine ; Panstruga, Ralph ; Booker, Fitzgerald L. ; Botella, José Ramón ; Molina, Antonio ; Ort, Donald R. ; Jones, Alan M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-df576c4917e605dfc84b5d1f10ab4957727d57c8b17cd7ef37bbab7978eabb273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Abscisic Acid - pharmacology</topic><topic>Agriculture</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - growth & development</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis - microbiology</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Biological and medical sciences</topic><topic>CELL BIOLOGY AND SIGNAL TRANSDUCTION</topic><topic>Flagellin - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungi</topic><topic>Glucose - pharmacology</topic><topic>GTP-Binding Protein beta Subunits - metabolism</topic><topic>Hypocotyls</topic><topic>Leaves</topic><topic>Models, Molecular</topic><topic>Mutagenesis, Site-Directed</topic><topic>Mutant Proteins - metabolism</topic><topic>Mutation - genetics</topic><topic>Phenotype</topic><topic>Phenotypes</topic><topic>Plant cells</topic><topic>Plant physiology and development</topic><topic>Plant roots</topic><topic>Plants</topic><topic>Plants, Genetically Modified</topic><topic>Protein Folding - drug effects</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Rice</topic><topic>Seedlings</topic><topic>Signal Transduction - drug effects</topic><topic>Surface Properties - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Kun</creatorcontrib><creatorcontrib>Frick-Cheng, Arwen</creatorcontrib><creatorcontrib>Trusov, Yuri</creatorcontrib><creatorcontrib>Delgado-Cerezo, Magdalena</creatorcontrib><creatorcontrib>Rosenthal, David M.</creatorcontrib><creatorcontrib>Lorek, Justine</creatorcontrib><creatorcontrib>Panstruga, Ralph</creatorcontrib><creatorcontrib>Booker, Fitzgerald L.</creatorcontrib><creatorcontrib>Botella, José Ramón</creatorcontrib><creatorcontrib>Molina, Antonio</creatorcontrib><creatorcontrib>Ort, Donald R.</creatorcontrib><creatorcontrib>Jones, Alan M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Kun</au><au>Frick-Cheng, Arwen</au><au>Trusov, Yuri</au><au>Delgado-Cerezo, Magdalena</au><au>Rosenthal, David M.</au><au>Lorek, Justine</au><au>Panstruga, Ralph</au><au>Booker, Fitzgerald L.</au><au>Botella, José Ramón</au><au>Molina, Antonio</au><au>Ort, Donald R.</au><au>Jones, Alan M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dissecting Arabidopsis Gβ Signal Transduction on the Protein Surface</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>2012-07-01</date><risdate>2012</risdate><volume>159</volume><issue>3</issue><spage>975</spage><epage>983</epage><pages>975-983</pages><issn>0032-0889</issn><issn>1532-2548</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>The heterotrimeric G-protein complex provides signal amplification and target specificity. The Arabidopsis (Arabidopsis thaliana) Gβ-subunit of this complex (AGB1) interacts with and modulates the activity of target cytoplasmic proteins. This specificity resides in the structure of the interface between AGB1 and its targets. Important surface residues of AGB1, which were deduced from a comparative evolutionary approach, were mutated to dissect AGB1-dependent physiological functions. Analysis of the capacity of these mutants to complement well-established phenotypes of Gβ-null mutants revealed AGB1 residues critical for specific AGB1-mediated biological processes, including growth architecture, pathogen resistance, stomata-mediated leaf-air gas exchange, and possibly photosynthesis. These findings provide promising new avenues to direct the finely tuned engineering of crop yield and traits.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Biologists</pub><pmid>22570469</pmid><doi>10.1104/pp.112.196337</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant physiology (Bethesda), 2012-07, Vol.159 (3), p.975-983 |
| issn | 0032-0889 1532-2548 1532-2548 |
| language | eng |
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| source | JSTOR Archival Journals and Primary Sources Collection; Oxford Journals Online |
| subjects | Abscisic Acid - pharmacology Agriculture Arabidopsis - genetics Arabidopsis - growth & development Arabidopsis - metabolism Arabidopsis - microbiology Arabidopsis Proteins - metabolism Biological and medical sciences CELL BIOLOGY AND SIGNAL TRANSDUCTION Flagellin - pharmacology Fundamental and applied biological sciences. Psychology Fungi Glucose - pharmacology GTP-Binding Protein beta Subunits - metabolism Hypocotyls Leaves Models, Molecular Mutagenesis, Site-Directed Mutant Proteins - metabolism Mutation - genetics Phenotype Phenotypes Plant cells Plant physiology and development Plant roots Plants Plants, Genetically Modified Protein Folding - drug effects Reactive Oxygen Species - metabolism Rice Seedlings Signal Transduction - drug effects Surface Properties - drug effects |
| title | Dissecting Arabidopsis Gβ Signal Transduction on the Protein Surface |
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