An embryonic DNA-binding protein specific for a region of the human IFN beta 1 promoter

Embryonal carcinoma (EC) cells are unable to make interferon in response to inducing agents. This block disappears after differentiation. We have found that nuclear extracts from undifferentiated P19 EC cells contain a DNA-binding activity which specifically recognizes a region within the human inte...

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Published in:Nucleic acids research 1988-11, Vol.16 (22), p.10575-10592
Main Authors: Haggarty, A, Ponton, A, Paterno, G D, Daigneault, L, Skup, D
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cell Line
Cell Nucleus
Cloning, Molecular
DNA-Binding Proteins - isolation & purification
DNA-Binding Proteins - metabolism
Genes
Humans
Interferon Type I - genetics
Mice
Molecular Sequence Data
Plasmids
Promoter Regions, Genetic
Restriction Mapping
Teratoma
Transfection
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container_end_page 10592
container_issue 22
container_start_page 10575
container_title Nucleic acids research
container_volume 16
creator Haggarty, A
Ponton, A
Paterno, G D
Daigneault, L
Skup, D
description Embryonal carcinoma (EC) cells are unable to make interferon in response to inducing agents. This block disappears after differentiation. We have found that nuclear extracts from undifferentiated P19 EC cells contain a DNA-binding activity which specifically recognizes a region within the human interferon-beta 1 promoter. This activity is absent from differentiated cell types, both of EC and non-EC origin. The binding of the factor in undifferentiated EC cells leads to dramatic changes in the overall protein binding pattern of the interferon promoter as compared with differentiated cells, and may be responsible for repression of the endogenous interferon-beta gene prior to differentiation.
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subjects Animals
Base Sequence
Cell Line
Cell Nucleus
Cloning, Molecular
DNA-Binding Proteins - isolation & purification
DNA-Binding Proteins - metabolism
Genes
Humans
Interferon Type I - genetics
Mice
Molecular Sequence Data
Plasmids
Promoter Regions, Genetic
Restriction Mapping
Teratoma
Transfection
title An embryonic DNA-binding protein specific for a region of the human IFN beta 1 promoter
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