The structure of nucleosomal core particles within transcribed and repressed gene regions

The arrangement of histones along DNA in nucleosomal core particles within transcribed heat shock gene (hsp 70) region and repressed insertion within ribosomal genes of Drosophila was analysed by using protein-DNA crosslinking methods combined with hybridization tests. In addition, two-dimensional g...

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Bibliographic Details
Published in:Nucleic acids research 1988-12, Vol.16 (23), p.11187-11205
Main Authors: STUDITSKY, V. M, BELYAVSKY, A. V, MELNIKOVA, A. F, MIRZABEKOV, A. D
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Chromatin - isolation & purification
Chromatin. Chromosome
Deoxyribonuclease I
Drosophila
Drosophila melanogaster
Drosophilidae
Fundamental and applied biological sciences. Psychology
Heat-Shock Proteins - genetics
Heat-Shock Proteins - isolation & purification
Histones - isolation & purification
Micrococcal Nuclease
Molecular and cellular biology
Molecular genetics
Nucleic Acid Hybridization
Nucleosomes - analysis
Repressor Proteins - genetics
Repressor Proteins - isolation & purification
Transcription Factors - genetics
Transcription, Genetic
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Summary:The arrangement of histones along DNA in nucleosomal core particles within transcribed heat shock gene (hsp 70) region and repressed insertion within ribosomal genes of Drosophila was analysed by using protein-DNA crosslinking methods combined with hybridization tests. In addition, two-dimensional gel electrophoresis was employed to compare the overall nucleosomal shape and the nucleosomal DNA size. The arrangement of histones along DNA and general compactness of nucleosomes were shown to be rather similar in transcriptionally active and inactive genomic regions. On the other hand, nucleosomes within transcriptionally active chromatin are characterized by a larger size of nucleosomal DNA produced by micrococcal nuclease digestion and some peculiarity in electrophoretic mobility.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/16.23.11187