cDNA and genomic cloning of yeast phenylalanine ammonia-lyase genes reveal genomic intron deletions

The authors have isolated cDNA and genomic clones of phenylalanine ammonia-lyase (PAL) from the yeast Rhodotorula rubra NRRL Y-15597. Five short introns were identified from a comparison of cDNA and genomic sequences. The gene encodes a protein of M sub(r) = 76,000 in agreement with the determined s...

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Bibliographic Details
Published in:Nucleic acids research 1988-12, Vol.16 (23), p.11382-11382
Main Authors: VASLET, C. A, STRAUSBERG, R. L, SYKES, A, LEVY, A, FILPULA, D
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Ammonia-Lyases - genetics
Base Sequence
Biological and medical sciences
Biological evolution
Chromosome Deletion
Cloning, Molecular
DNA - isolation & purification
Fundamental and applied biological sciences. Psychology
genes
Genetics of eukaryotes. Biological and molecular evolution
Introns
Mitosporic Fungi - enzymology
Molecular Sequence Data
phenylalanine ammonia-lyase
Phenylalanine Ammonia-Lyase - genetics
Rhodotorula - enzymology
Rhodotorula - genetics
Rhodotorula rubra
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Summary:The authors have isolated cDNA and genomic clones of phenylalanine ammonia-lyase (PAL) from the yeast Rhodotorula rubra NRRL Y-15597. Five short introns were identified from a comparison of cDNA and genomic sequences. The gene encodes a protein of M sub(r) = 76,000 in agreement with the determined subunit molecular weight.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/16.23.11382