Both subunits of rat liver ferritin are regulated at a translational level by iron induction

A few hours after administering iron to rats, liver ferritin synthesis increases several fold. However, Northern blot analysis with cDNA probes for ferritin light (L) and heavy (H) subunlt mRNAs failed to show an increase in total population of either messenger. Cytoplasmic distribution of ferritin...

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Bibliographic Details
Published in:Nucleic acids research 1986-01, Vol.14 (2), p.915-927
Main Authors: Aziz, Nazneen, Munro, Hamish N.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cytoplasm - metabolism
DNA - analysis
Ferritins - biosynthesis
Ferritins - genetics
Fundamental and applied biological sciences. Psychology
Iron - pharmacology
Kinetics
Liver - metabolism
Macromolecular Substances
Male
Molecular and cellular biology
Molecular genetics
Nucleic Acid Hybridization
Plasmids
Protein Biosynthesis - drug effects
Rats
Rats, Inbred Strains
RNA - genetics
RNA - isolation & purification
Translation. Translation factors. Protein processing
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Summary:A few hours after administering iron to rats, liver ferritin synthesis increases several fold. However, Northern blot analysis with cDNA probes for ferritin light (L) and heavy (H) subunlt mRNAs failed to show an increase in total population of either messenger. Cytoplasmic distribution of ferritin messages was therefore investigated in control and iron administered rats killed at 3.5 hours. The liver post-mitochondrial supernatant was fractionated on a sucrose gradient to separate polyribosomea, monosomes, ribosomal subunits and cell sap. RNA extracted from each fraction and analyzed using Northern blotting showed that 65% of the total mRNA population for each subunit was present in the cell sap of control rats, presumably as mRNP particles since ribosomal RNA was absent from this fraction. After iron administration, these reserves of free mRNA were recruited onto the polysomes, reducing the free mRNA pool to 15% of the total. We interpret this to be due to activation of blocked ferritin messages on entry of iron Into the cell.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/14.2.915