Lipopolysaccharide induction of autophagy is associated with enhanced bactericidal activity in Dictyostelium discoideum

► Autophagy is induced in Dictyostelium discoideum exposed to Salmonella typhimurium and Klebsiella pneumoniae. ► LPS induces autophagosomal maturation in D. discoideum cells exposed to Staphylococcus aureus. ► Rapamycin-induced autophagy enhances bactericidal activity in D. discoideum. ► LPS-enhanc...

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Published in:Biochemical and biophysical research communications 2012-06, Vol.422 (3), p.417-422
Main Authors: Pflaum, Katherine, Gerdes, Kimberly, Yovo, Kossi, Callahan, Jennifer, Snyder, Michelle L.D.
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Autophagy
Autophagy - drug effects
Autophagy - immunology
Dictyostelium - immunology
Dictyostelium - microbiology
Dictyostelium discoideum
Innate immunity
Lipopolysaccharides - immunology
LPS
Lysosome
Pattern recognition
Phagocytosis
Phagosomes - immunology
Phagosomes - microbiology
Sirolimus - pharmacology
Staphylococcus aureus
Staphylococcus aureus - immunology
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Summary:► Autophagy is induced in Dictyostelium discoideum exposed to Salmonella typhimurium and Klebsiella pneumoniae. ► LPS induces autophagosomal maturation in D. discoideum cells exposed to Staphylococcus aureus. ► Rapamycin-induced autophagy enhances bactericidal activity in D. discoideum. ► LPS-enhanced bacterial clearance in D. discoideum is dependent on Atg1 and Atg9. Innate immune cells respond to microbial invaders using pattern recognition receptors that detect conserved microbial patterns. Among the cellular processes stimulated downstream of pattern recognition machinery is the initiation of autophagy, which plays protective roles against intracellular microbes. We have shown recently that Dictyostelium discoideum, which takes up bacteria for nutritive purposes, may employ pattern recognition machinery to respond to bacterial prey, as D. discoideum cells upregulate bactericidal activity upon stimulation by lipopolysaccharide (LPS). Here we extend these findings, showing that LPS treatment leads to induction of autophagosomal maturation in cells responding to the bacteria Staphylococcus aureus. Cells treated with the autophagy-inducing drug rapamycin clear internalized bacteria at an accelerated rate, while LPS-enhanced clearance of bacteria is reduced in cells deficient for the autophagy-related genes atg1 and atg9. These findings link microbial pattern recognition with autophagy in the social amoeba D. discoideum.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2012.05.006