Activation of oral trigeminal neurons by fatty acids is dependent upon intracellular calcium

The chemoreception of dietary fat in the oral cavity has largely been attributed to activation of the somatosensory system that conveys the textural properties of fat. However, the ability of fatty acids, which are believed to represent the proximate stimulus for fat taste, to stimulate rat trigemin...

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Bibliographic Details
Published in:Pflügers Archiv 2012-08, Vol.464 (2), p.227-237
Main Authors: Yu, Tian, Shah, Bhavik P., Hansen, Dane R., Park-York, MieJung, Gilbertson, Timothy A.
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Calcium Signaling - drug effects
Calcium Signaling - physiology
Cell Biology
Cells, Cultured
Fatty Acids - pharmacology
Herpesvirus 1, Suid
Human Physiology
Linoleic Acid - pharmacology
Male
Membrane Potentials - drug effects
Membrane Potentials - physiology
Molecular Medicine
Neurons - drug effects
Neurons - metabolism
Neurons - physiology
Neurosciences
Rats
Rats, Sprague-Dawley
Receptors
Sensory Physiology
Taste - physiology
Trigeminal Ganglion - drug effects
Trigeminal Ganglion - metabolism
Trigeminal Ganglion - physiology
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Summary:The chemoreception of dietary fat in the oral cavity has largely been attributed to activation of the somatosensory system that conveys the textural properties of fat. However, the ability of fatty acids, which are believed to represent the proximate stimulus for fat taste, to stimulate rat trigeminal neurons has remained unexplored. Here, we found that several free fatty acids are capable of activating trigeminal neurons with different kinetics. Further, a polyunsaturated fatty acid, linoleic acid (LA), activates trigeminal neurons by increasing intracellular calcium concentration and generating depolarizing receptor potentials. Ion substitution and pharmacological approaches reveal that intracellular calcium store depletion is crucial for LA-induced signaling in a subset of trigeminal neurons. Using pseudorabies virus (PrV) as a live cell tracer, we identified a subset of lingual nerve-innervated trigeminal neurons that respond to different subsets of fatty acids. Quantitative real-time PCR of several transient receptor potential channel markers in individual neurons validated that PrV labeled a subset but not the entire population of lingual-innervated trigeminal neurons. We further confirmed that the LA-induced intracellular calcium rise is exclusively coming from the release of calcium stores from the endoplasmic reticulum in this subset of lingual nerve-innervated trigeminal neurons.
ISSN:0031-6768
1432-2013
DOI:10.1007/s00424-012-1116-9