Secretory Pathway Research: The More Experimental Systems the Better

Transient gene expression, in plant protoplasts or specific plant tissues, is a key technique in plant molecular cell biology, aimed at exploring gene products and their modifications to examine functional subdomains, their interactions with other biomolecules, and their subcellular localization. He...

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Bibliographic Details
Published in:The Plant cell 2012-04, Vol.24 (4), p.1316-1326
Main Authors: Denecke, Jurgen, Aniento, Femando, Frigerio, Lorenzo, Hawes, Chris, Hwang, Inhwan, Mathur, Jaideep, Neuhaus, Jean-Marc, Robinson, David G.
Format: Article
Language:English
Subjects:
Biological Transport
Cell walls
DNA
Fluorescent Dyes - metabolism
Freight
Organelles
PERSPECTIVE
Plant cells
Plant Leaves - metabolism
Plant Leaves - microbiology
Plant tissues
Plants
Proteins
Protoplasts
Protoplasts - metabolism
Protoplasts - ultrastructure
Receptors
Secretory Pathway
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Summary:Transient gene expression, in plant protoplasts or specific plant tissues, is a key technique in plant molecular cell biology, aimed at exploring gene products and their modifications to examine functional subdomains, their interactions with other biomolecules, and their subcellular localization. Here, we highlight some of the major advantages and potential pitfalls of the most commonly used transient gene expression models and illustrate how ectopie expression and the use of dominant mutants can provide insights into protein function.
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.112.096362