Transcriptional control of yeast ribosomal protein synthesis during carbon-source upshift

Shifting a yeast culture from an ethanol-based medium to a glucose-based medium causes a coordinate increase of the cellular levels of ribosomal protein mRNAs by about a factor 4 within 30 min. Making use of hybrid genes encompassing different portions of the 5'-flanking region of the L25-gene,...

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Bibliographic Details
Published in:Nucleic acids research 1987-12, Vol.15 (24), p.10133-10144
Main Authors: HERRUER, M. H, MAGER, W. H, WOUDT, L. P, NIEUWINT, R. T. M, WASSENAAR, G. M, GROENEVELD, P, PLANTA, R. J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Ethanol - metabolism
Fundamental and applied biological sciences. Psychology
Fungal Proteins - biosynthesis
Gene Expression Regulation
Glucose - metabolism
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Microbiology
Multigene Family
Mycology
Recombinant Fusion Proteins - genetics
Regulatory Sequences, Nucleic Acid
Ribosomal Proteins - biosynthesis
RNA, Fungal - genetics
RNA, Messenger - genetics
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Transcription, Genetic
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Summary:Shifting a yeast culture from an ethanol-based medium to a glucose-based medium causes a coordinate increase of the cellular levels of ribosomal protein mRNAs by about a factor 4 within 30 min. Making use of hybrid genes encompassing different portions of the 5'-flanking region of the L25-gene, we could show that the increase in mRNAs is a transcriptional event, mediated through DNA sequences upstream of the ribosomal protein (rp) genes. Further analysis revealed that sequence elements are involved that many rp-genes have in common and that previously were identified as transcription activation sites (RPG-boxes or UASrpg). Using appropriate deletion mutants of the fusion genes we could demonstrate that a single RPG-box is sufficient for the transcriptional upshift. In addition, both copy genes encoding rp28 which differ considerably in their extent of transcriptional activity, show the upshift effect in a proportional manner. Definite proof for the role of the UASrpg in nutritional regulation was obtained by examining the effect of a synthetic RPG-box on transcription.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/15.24.10133