Nucleotide sequence of a full-length infectious clone of the Indonesian strain of tomato apical stunt viroid (TASV)

Using a two primer method the authors have obtained full-length monomeric clones of an Indonesian isolate of tomato apical stunt viroid (TASV). Infectivity of the cloned sequences was confirmed, by inoculation of double stranded recombinant plasmid DNA as shown previously for potato spindle tuber vi...

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Bibliographic Details
Published in:Nucleic acids research 1987-12, Vol.15 (24), p.10597-10597
Main Authors: CANDRESSE, T, SMITH, D, DIENER, T. O
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Cloning, Molecular
Fundamental and applied biological sciences. Psychology
Genetics
Microbiology
Molecular Sequence Data
Plants - microbiology
tomato apical stunt viroid
Viroids - genetics
Virology
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Summary:Using a two primer method the authors have obtained full-length monomeric clones of an Indonesian isolate of tomato apical stunt viroid (TASV). Infectivity of the cloned sequences was confirmed, by inoculation of double stranded recombinant plasmid DNA as shown previously for potato spindle tuber viroid (PSTV). The complete nucleotide sequence of two independent infectious clones was determined using the chemical cleavage method of Maxam and Gilbert. No heterogeneity was found when comparing the sequences derived from the two clones.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/15.24.10597