Bond-selective imaging of deep tissue through the optical window between 1600 and 1850 nm

We report the employment of an optical window between 1600 nm and 1850 nm for bond‐selective deep tissue imaging through harmonic vibrational excitation and acoustic detection of resultant pressure waves. In this window where a local minimum of water absorption resides, we found a 5 times enhancemen...

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Bibliographic Details
Published in:Journal of biophotonics 2012-01, Vol.5 (1), p.25-32
Main Authors: Wang, Pu, Wang, Han-Wei, Sturek, Michael, Cheng, Ji-Xin
Format: Article
Language:English
Subjects:
Absorption
Acoustics
Animals
atherosclerosis
Atherosclerosis - diagnosis
Atherosclerosis - pathology
Contrast Media
deep tissue imaging
Goats
Imaging, Three-Dimensional
Lipids - analysis
Molecular Imaging - methods
optical window
Optics and Photonics - methods
Phantoms, Imaging
photoacoustic imaging
Proteins - analysis
Rats
Rats, Sprague-Dawley
Swine
Vibration
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Summary:We report the employment of an optical window between 1600 nm and 1850 nm for bond‐selective deep tissue imaging through harmonic vibrational excitation and acoustic detection of resultant pressure waves. In this window where a local minimum of water absorption resides, we found a 5 times enhancement of photoacoustic signal by first overtone excitation of the methylene group CH2 at 1730 nm, compared to the second overtone excitation at 1210 nm. The enhancement allows 3D mapping of intramuscular fat with improved contrast and of lipid deposition inside an atherosclerotic artery wall in the presence of blood. Moreover, lipid and protein are differentiated based on the first overtone absorption profiles of CH2 and methyl group CH3 in this window. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
ISSN:1864-063X
1864-0648
DOI:10.1002/jbio.201100102