Isolation, structure and expression of mammalian genes for histidyl-tRNA synthetase

A full length cDNA clone that codes for human histidyl-tRNA synthetase (HRS) and cDNA clones that span the full length transcript of hamster HRS have been isolated. The full length human HRS cDNA was expressed after transfection into Cos 1 cells and a CHO ts mutant defective in the gene for HRS. The...

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Bibliographic Details
Published in:Nucleic acids research 1987-04, Vol.15 (8), p.3349-3367
Main Authors: TSUI, F. W. L, SIMINOVITCH, L
Format: Article
Language:English
Subjects:
amino acid sequence
Amino Acyl-tRNA Synthetases - genetics
Animals
Bacterial Proteins - genetics
Base Sequence
Biological and medical sciences
cDNA
Cricetinae
DNA - genetics
DNA, Recombinant
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Fungal Proteins - genetics
gene expression
Genes
Genes, Bacterial
Genes, Fungal
Genes. Genome
hamsters
histidine-tRNA ligase
Histidine-tRNA Ligase - genetics
Humans
man
Molecular and cellular biology
Molecular genetics
nucleotide sequence
Saccharomyces cerevisiae - genetics
Sequence Homology, Nucleic Acid
Species Specificity
transfection
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Summary:A full length cDNA clone that codes for human histidyl-tRNA synthetase (HRS) and cDNA clones that span the full length transcript of hamster HRS have been isolated. The full length human HRS cDNA was expressed after transfection into Cos 1 cells and a CHO ts mutant defective in the gene for HRS. The complete nucleotide sequence of the hamster and human gene were obtained and extensive homologies were observed in three regions on comparing these sequences between themselves and with the sequence of HRS derived from yeast. These results provide unequivocal evidence that we have indeed cloned the hamster and human gene for HRS. Three overlapping phage recombinants containing the complete hamster chromosomal gene for HRS have also been isolated. The genomic HRS is divided into 13 exons. The precise locations of each of the 5' and 3' exon-intron boundaries were defined by sequencing the appropriate regions of the cloned genomic DNA and aligning them with the sequence of HRS cDNAs. These studies provide the basis for future structural and functional analysis of the gene for HRS. In particular, it will be of interest to examine if different exons of HRS correlate to different domains of the HRS polypeptide.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/15.8.3349