Comparison of the conformation of an oligonucleotide containing a central G-T base pair with the non-mismatch sequence by proton NMR

We have recorded NOESY spectra of two non-selfcomplementary undecanucleotide duplexes. From the observed NOEs we do not detect any significant distortion of the helix when a G-C pair is replaced by a G-T pair and the normal interresidue connectivities can be followed through the mismatch site. We co...

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Bibliographic Details
Published in:Nucleic acids research 1987-04, Vol.15 (8), p.3397-3409
Main Authors: QUIGNARD, E, FAZAKERLEY, G. V, VAN DER MAREL, G, VAN BOOM, J. H, GUSCHLBAUER, W
Format: Article
Language:English
Subjects:
Adenosine
Base Composition
Base Sequence
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Magnetic Resonance Spectroscopy - methods
Molecular biophysics
Nucleic Acid Conformation
Oligodeoxyribonucleotides
Protons
Structure in molecular biology
Tridimensional structure
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Summary:We have recorded NOESY spectra of two non-selfcomplementary undecanucleotide duplexes. From the observed NOEs we do not detect any significant distortion of the helix when a G-C pair is replaced by a G-T pair and the normal interresidue connectivities can be followed through the mismatch site. We conclude that the 2D spectra of the non-exchangeable protons do not allow differentiation between a wobble or rare tautomer form for the mismatch. NOE measurements in H2O, however, clearly show that the mismatch adopts a wobble structure and give information on the hydration in the minor groove for the G-T base pair which is embedded between two A-T base pairs in the sequence.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/15.8.3397