Organ-specific and dosage-dependent expression of a leaf/stem specific gene from potato after tagging and transfer into potato and tobacco plants

ST-LS1, a single copy gene from potato displaying a leaf/stem specific gene expression, was tagged by an exon modification and introduced into both potato and tobacco cells using Agrobacterium vectors. After regeneration of whole plants, the expression of the tagged gene was analyzed with respect to...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1987-04, Vol.15 (8), p.3479-3491
Main Authors: STOCKHAUS, J, ECKES, P, BLAU, A, SCHELL, J, WILLMITZER, L
Format: Article
Language:English
Subjects:
Agrobacterium
Biological and medical sciences
Biotechnology
DNA, Recombinant
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Genes
Genetic engineering
Genetic technics
Genetic Vectors
Methods. Procedures. Technologies
Nicotiana - genetics
Organ Specificity
Plants, Toxic
Solanum tuberosum
Solanum tuberosum - genetics
Transformation, Genetic
Vectors (cloning, transfer, expression). Insertion sequences and transposons
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:ST-LS1, a single copy gene from potato displaying a leaf/stem specific gene expression, was tagged by an exon modification and introduced into both potato and tobacco cells using Agrobacterium vectors. After regeneration of whole plants, the expression of the tagged gene was analyzed with respect to its organ specificity and compared to the expression of the corresponding resident gene. The expression of the transferred gene in transgenic plants closely followed the expression of the resident gene. No marked influence of the plant species serving as host was observed. The level of expression of the introduced gene varied by a factor of at least 100 in independent transformants when normalized to the expression of the resident gene. Southern analysis performed on the transformed plants indicated a correlation between copy number of the introduced gene and its expression level. The activity of the tagged gene as well as of the resident gene was significantly inhibited by treatment of the transgenic plants with the herbicide norfluorazon, indicating that this gene activity is dependent on the presence of functional chloroplasts in the leaves.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/15.8.3479