Competition studies with repressors and activators of viral enhancer function in F9 mouse embryonal carcinoma cells

DNA competition studies have been used to investigate the presence of a repressor of viral enhancer function in F9 mouse embryonal carcinoma cells. The complete polyoma virus enhancer region, cotransfected into F9 cells with the SV40 promoter/enhancer attached to a chloramphenicol acetyl transferase...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1987-05, Vol.15 (10), p.4307-4324
Main Authors: SLEIGH, M. J, LOCKETT, T. J, LEWY, D
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Differentiation - drug effects
Cell Line
Cyclic AMP - physiology
DNA, Bacterial - genetics
DNA, Bacterial - pharmacology
DNA, Recombinant - pharmacology
Enhancer Elements, Genetic - drug effects
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation - drug effects
Genes, Regulator - drug effects
Genes, Synthetic
Genes, Viral - drug effects
Mice
Molecular and cellular biology
Molecular genetics
Polyomavirus - genetics
Promoter Regions, Genetic
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - genetics
Repressor Proteins - metabolism
Simian virus 40 - genetics
Teratoma - genetics
Teratoma - pathology
Transcription Factors - metabolism
Transfection
Tretinoin - pharmacology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:DNA competition studies have been used to investigate the presence of a repressor of viral enhancer function in F9 mouse embryonal carcinoma cells. The complete polyoma virus enhancer region, cotransfected into F9 cells with the SV40 promoter/enhancer attached to a chloramphenicol acetyl transferase marker gene, induced a small increase in pSV2CAT expression. This can be explained by preferential but weak binding by polyoma sequences of a molecule repressing pSV2CAT transcription. Repressor activity substantially disappeared when the cells were induced to differentiate by retinoic acid. Repressor binding was localised to one half of the polyoma enhancer, but was lost on further fragmentation of this region. It appears that multiple sequence elements may be required for repressor binding and that these are at least partially separable from the complement of elements binding enhancer activating molecules.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/15.10.4307