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Competition studies with repressors and activators of viral enhancer function in F9 mouse embryonal carcinoma cells
DNA competition studies have been used to investigate the presence of a repressor of viral enhancer function in F9 mouse embryonal carcinoma cells. The complete polyoma virus enhancer region, cotransfected into F9 cells with the SV40 promoter/enhancer attached to a chloramphenicol acetyl transferase...
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| Published in: | Nucleic acids research 1987-05, Vol.15 (10), p.4307-4324 |
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| container_end_page | 4324 |
| container_issue | 10 |
| container_start_page | 4307 |
| container_title | Nucleic acids research |
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| creator | SLEIGH, M. J LOCKETT, T. J LEWY, D |
| description | DNA competition studies have been used to investigate the presence of a repressor of viral enhancer function in F9 mouse embryonal carcinoma cells. The complete polyoma virus enhancer region, cotransfected into F9 cells with the SV40 promoter/enhancer attached to a chloramphenicol acetyl transferase marker gene, induced a small increase in pSV2CAT expression. This can be explained by preferential but weak binding by polyoma sequences of a molecule repressing pSV2CAT transcription. Repressor activity substantially disappeared when the cells were induced to differentiate by retinoic acid. Repressor binding was localised to one half of the polyoma enhancer, but was lost on further fragmentation of this region. It appears that multiple sequence elements may be required for repressor binding and that these are at least partially separable from the complement of elements binding enhancer activating molecules. |
| doi_str_mv | 10.1093/nar/15.10.4307 |
| format | article |
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It appears that multiple sequence elements may be required for repressor binding and that these are at least partially separable from the complement of elements binding enhancer activating molecules.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/15.10.4307</identifier><identifier>PMID: 3035489</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Animals ; Biological and medical sciences ; Cell Differentiation - drug effects ; Cell Line ; Cyclic AMP - physiology ; DNA, Bacterial - genetics ; DNA, Bacterial - pharmacology ; DNA, Recombinant - pharmacology ; Enhancer Elements, Genetic - drug effects ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation - drug effects ; Genes, Regulator - drug effects ; Genes, Synthetic ; Genes, Viral - drug effects ; Mice ; Molecular and cellular biology ; Molecular genetics ; Polyomavirus - genetics ; Promoter Regions, Genetic ; Recombinant Fusion Proteins - biosynthesis ; Recombinant Fusion Proteins - genetics ; Repressor Proteins - metabolism ; Simian virus 40 - genetics ; Teratoma - genetics ; Teratoma - pathology ; Transcription Factors - metabolism ; Transfection ; Tretinoin - pharmacology</subject><ispartof>Nucleic acids research, 1987-05, Vol.15 (10), p.4307-4324</ispartof><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-9738e5ef20eea4bd7f12fa9a6b0e732f3df6741a94202672e2d4b513733321c33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC340849/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC340849/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7464398$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3035489$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SLEIGH, M. J</creatorcontrib><creatorcontrib>LOCKETT, T. J</creatorcontrib><creatorcontrib>LEWY, D</creatorcontrib><title>Competition studies with repressors and activators of viral enhancer function in F9 mouse embryonal carcinoma cells</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>DNA competition studies have been used to investigate the presence of a repressor of viral enhancer function in F9 mouse embryonal carcinoma cells. The complete polyoma virus enhancer region, cotransfected into F9 cells with the SV40 promoter/enhancer attached to a chloramphenicol acetyl transferase marker gene, induced a small increase in pSV2CAT expression. This can be explained by preferential but weak binding by polyoma sequences of a molecule repressing pSV2CAT transcription. Repressor activity substantially disappeared when the cells were induced to differentiate by retinoic acid. Repressor binding was localised to one half of the polyoma enhancer, but was lost on further fragmentation of this region. It appears that multiple sequence elements may be required for repressor binding and that these are at least partially separable from the complement of elements binding enhancer activating molecules.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Line</subject><subject>Cyclic AMP - physiology</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - pharmacology</subject><subject>DNA, Recombinant - pharmacology</subject><subject>Enhancer Elements, Genetic - drug effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Genes, Regulator - drug effects</subject><subject>Genes, Synthetic</subject><subject>Genes, Viral - drug effects</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Polyomavirus - genetics</subject><subject>Promoter Regions, Genetic</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Repressor Proteins - metabolism</subject><subject>Simian virus 40 - genetics</subject><subject>Teratoma - genetics</subject><subject>Teratoma - pathology</subject><subject>Transcription Factors - metabolism</subject><subject>Transfection</subject><subject>Tretinoin - pharmacology</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNpVkc1v1DAQxa0KVJbClRuSD6i3bP2VOD5wQCtKkSpxgbPlOOOuUWIvtrOo_z1Ou1rRkz16vzee8UPoAyVbShS_CSbd0Lbet4ITeYE2lHesEapjr9CGcNI2lIj-DXqb829CqKCtuESXnPBW9GqD8i7OByi--BhwLsvoIeO_vuxxgkOCnGPK2IQRG1v80ZS1jA4ffTIThrA3wULCbgn2qYMP-FbhOS4ZMMxDeoyhctYk60OcDbYwTfkdeu3MlOH96bxCv26__tzdNfc_vn3ffblvrBCiNEryHlpwjAAYMYzSUeaMMt1AQHLm-Og6KahRghHWSQZsFENLueScM2o5v0Kfn_selmGG0UIodWp9SH426VFH4_VLJfi9fohHzQXphar-65M_xT8L5KJnn9cNTIC6oaZC9m1PZQW3z6BNMecE7vwGJXpNSdeUNG3Xck2pGj7-P9kZP8VS9U8n3WRrJpfqN_t8xqToBFc9_wdYpJ3y</recordid><startdate>19870526</startdate><enddate>19870526</enddate><creator>SLEIGH, M. 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J ; LEWY, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-9738e5ef20eea4bd7f12fa9a6b0e732f3df6741a94202672e2d4b513733321c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Line</topic><topic>Cyclic AMP - physiology</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Bacterial - pharmacology</topic><topic>DNA, Recombinant - pharmacology</topic><topic>Enhancer Elements, Genetic - drug effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Genes, Regulator - drug effects</topic><topic>Genes, Synthetic</topic><topic>Genes, Viral - drug effects</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Polyomavirus - genetics</topic><topic>Promoter Regions, Genetic</topic><topic>Recombinant Fusion Proteins - biosynthesis</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Repressor Proteins - metabolism</topic><topic>Simian virus 40 - genetics</topic><topic>Teratoma - genetics</topic><topic>Teratoma - pathology</topic><topic>Transcription Factors - metabolism</topic><topic>Transfection</topic><topic>Tretinoin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SLEIGH, M. J</creatorcontrib><creatorcontrib>LOCKETT, T. 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J</au><au>LEWY, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Competition studies with repressors and activators of viral enhancer function in F9 mouse embryonal carcinoma cells</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1987-05-26</date><risdate>1987</risdate><volume>15</volume><issue>10</issue><spage>4307</spage><epage>4324</epage><pages>4307-4324</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>DNA competition studies have been used to investigate the presence of a repressor of viral enhancer function in F9 mouse embryonal carcinoma cells. The complete polyoma virus enhancer region, cotransfected into F9 cells with the SV40 promoter/enhancer attached to a chloramphenicol acetyl transferase marker gene, induced a small increase in pSV2CAT expression. This can be explained by preferential but weak binding by polyoma sequences of a molecule repressing pSV2CAT transcription. Repressor activity substantially disappeared when the cells were induced to differentiate by retinoic acid. Repressor binding was localised to one half of the polyoma enhancer, but was lost on further fragmentation of this region. It appears that multiple sequence elements may be required for repressor binding and that these are at least partially separable from the complement of elements binding enhancer activating molecules.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>3035489</pmid><doi>10.1093/nar/15.10.4307</doi><tpages>18</tpages><oa>free_for_read</oa></addata></record> |
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| source | Open Access: PubMed Central; Oxford University Press:Jisc Collections:Oxford Journal Archive: Access period 2024-2025 |
| subjects | Animals Biological and medical sciences Cell Differentiation - drug effects Cell Line Cyclic AMP - physiology DNA, Bacterial - genetics DNA, Bacterial - pharmacology DNA, Recombinant - pharmacology Enhancer Elements, Genetic - drug effects Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation - drug effects Genes, Regulator - drug effects Genes, Synthetic Genes, Viral - drug effects Mice Molecular and cellular biology Molecular genetics Polyomavirus - genetics Promoter Regions, Genetic Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics Repressor Proteins - metabolism Simian virus 40 - genetics Teratoma - genetics Teratoma - pathology Transcription Factors - metabolism Transfection Tretinoin - pharmacology |
| title | Competition studies with repressors and activators of viral enhancer function in F9 mouse embryonal carcinoma cells |
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