Biocompatibility and biofilm inhibition of N , N -hexyl,methyl-polyethylenimine bonded to Boston Keratoprosthesis materials

Abstract The biocompatibility and antibacterial properties of N , N -hexyl,methyl-polyethylenimine (HMPEI) covalently attached to the Boston Keratoprosthesis (B-KPro) materials was evaluated. By means of confocal and electron microscopies, we observed that HMPEI-derivatized materials exert an inhibi...

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Bibliographic Details
Published in:Biomaterials 2011-12, Vol.32 (34), p.8783-8796
Main Authors: Behlau, Irmgard, Mukherjee, Koushik, Todani, Amit, Tisdale, Ann S, Cade, Fabiano, Wang, Liqiang, Leonard, Elizabeth M, Zakka, Fouad R, Gilmore, Michael S, Jakobiec, Frederick A, Dohlman, Claes H, Klibanov, Alexander M
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Antibacterial
Biocompatible Materials - chemistry
Biocompatible Materials - pharmacology
Biofilms - drug effects
Cornea - cytology
Cornea - drug effects
Corneal cytotoxicity
Dentistry
Keratoprosthesis
PMMA
Polyethyleneimine - chemistry
Polyethyleneimine - pharmacology
Polyethylenimine (PEI)
Polymethyl Methacrylate - chemistry
Polymethyl Methacrylate - pharmacology
Prostheses and Implants
Rabbits
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus aureus - physiology
Titanium
Titanium - chemistry
Titanium - pharmacology
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Summary:Abstract The biocompatibility and antibacterial properties of N , N -hexyl,methyl-polyethylenimine (HMPEI) covalently attached to the Boston Keratoprosthesis (B-KPro) materials was evaluated. By means of confocal and electron microscopies, we observed that HMPEI-derivatized materials exert an inhibitory effect on biofilm formation by Staphylococcus aureus clinical isolates, as compared to the parent poly(methyl methacrylate) (PMMA) and titanium. There was no additional corneal epithelial cell cytotoxicity of HMPEI-coated PMMA compared to that of control PMMA in tissue cultures in vitro . Likewise, no toxicity or adverse reactivity was detected with HMPEI-derivatized PMMA or titanium compared to those of the control materials after intrastromal or anterior chamber implantation in rabbits in vivo.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2011.08.010