Single-drop optimization of protein crystallization

A completely new crystal‐growth device has been developed that permits charting a course across the phase diagram to produce crystalline samples optimized for diffraction experiments. The utility of the device is demonstrated for the production of crystals for the traditional X‐ray diffraction data‐...

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Bibliographic Details
Published in:Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2012-08, Vol.68 (8), p.994-998
Main Authors: Meyer, Arne, Dierks, Karsten, Hilterhaus, Dierk, Klupsch, Thomas, Mühlig, Peter, Kleesiek, Jens, Schöpflin, Robert, Einspahr, Howard, Hilgenfeld, Rolf, Betzel, Christian
Format: Article
Language:English
Subjects:
Animals
Crotalid Venoms - analysis
Crotalus
crystal growth
Crystal structure
Crystallization
Crystallography, X-Ray - instrumentation
Crystallography, X-Ray - methods
Crystals
Devices
Diffraction
Laboratory Communications
Marantaceae - chemistry
Microorganisms
Optimization
Phase diagrams
Plant Proteins - analysis
Ribosome Inactivating Proteins, Type 2 - analysis
single-drop optimization
Synchrotrons
Toxins, Biological - analysis
Viscum album - chemistry
Xtal-Controller
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Summary:A completely new crystal‐growth device has been developed that permits charting a course across the phase diagram to produce crystalline samples optimized for diffraction experiments. The utility of the device is demonstrated for the production of crystals for the traditional X‐ray diffraction data‐collection experiment, of microcrystals optimal for data‐collection experiments at a modern microbeam insertion‐device synchrotron beamline and of nanocrystals required for data collection on an X‐ray laser beamline.
ISSN:1744-3091
1744-3091
2053-230X
DOI:10.1107/S1744309112024074