Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls

Here we discuss proteomic analyses of whole cell preparations of the mosquito stages of malaria parasite development (i.e. gametocytes, microgamete, ookinete, oocyst and sporozoite) of Plasmodium berghei. We also include critiques of the proteomes of two cell fractions from the purified ookinete, na...

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Bibliographic Details
Published in:Parasitology 2012-08, Vol.139 (9), p.1131-1145
Main Authors: WASS, M. N., STANWAY, R., BLAGBOROUGH, A. M., LAL, K., PRIETO, J. H., RAINE, D., STERNBERG, M. J. E., TALMAN, A. M., TOMLEY, F., YATES, J., SINDEN, R. E.
Format: Article
Language:English
Subjects:
Animals
Aquatic insects
Biological and medical sciences
Culicidae
Culicidae - parasitology
Female
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Human protozoal diseases
Infectious diseases
Insect Vectors - parasitology
Invertebrates
Life Cycle Stages
Malaria
Malaria - parasitology
Male
Medical sciences
Mosquitoes
Parasites
Parasitic diseases
Plasmodium berghei
Plasmodium berghei - growth & development
Plasmodium berghei - metabolism
Proteomics - methods
Protozoal diseases
Protozoan Proteins - metabolism
Vector-borne diseases
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Summary:Here we discuss proteomic analyses of whole cell preparations of the mosquito stages of malaria parasite development (i.e. gametocytes, microgamete, ookinete, oocyst and sporozoite) of Plasmodium berghei. We also include critiques of the proteomes of two cell fractions from the purified ookinete, namely the micronemes and cell surface. Whereas we summarise key biological interpretations of the data, we also try to identify key methodological constraints we have met, only some of which we were able to resolve. Recognising the need to translate the potential of current genome sequencing into functional understanding, we report our efforts to develop more powerful combinations of methods for the in silico prediction of protein function and location. We have applied this analysis to the proteome of the male gamete, a cell whose very simple structural organisation facilitated interpretation of data. Some of the in silico predictions made have now been supported by ongoing protein tagging and genetic knockout studies. We hope this discussion may assist future studies.
ISSN:0031-1820
1469-8161
1469-8161
DOI:10.1017/S0031182012000133